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2018 ; 12
(1
): 401-411
Nephropedia Template TP
gab.com Text
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Restoration of the basement membrane after wounding: a hallmark of young human
skin altered with aging
#MMPMID29086203
Fisher G
; Rittié L
J Cell Commun Signal
2018[Mar]; 12
(1
): 401-411
PMID29086203
show ga
In skin, the basement membrane at the dermal-epidermal junction (DEJ-BM) is an
important structure that tightly binds the epidermis to the dermis, and acts as a
permeability barrier that controls exchange of macromolecules. Repair of the
DEJ-BM during wound healing is important for restoration of skin functional
properties after wounding. Here, we used a CO(2) laser to perform partial
thickness wounds in human volunteers, and directly compared wound repair in
healthy young and aged individuals, focusing on the DEJ-BM. Our results show that
the DEJ-BM is restored within four weeks after partial thickness wounds in young
adults. We identified laminin-?2 as preferred substrate for keratinocytes during
reepithelialization of partial thickness human wounds. Laminin-?2 is expressed
continuously by migrating keratinocytes during reepithelialization, whereas
collagen IV and collagen VII are deposited after wound closure. In contrast, our
study shows that the DEJ-BM restoration following wounding is deficient in
elderly individuals. Specifically, COL7A2 was barely increased during wound
repair in aged skin and, as a result, the DEJ-BM in elderly skin was not restored
and showed abnormal structure. Our data suggest that ameliorating the quality of
the DEJ-BM restoration is a promising therapeutic approach to improve the quality
of repaired skin in the elderly.