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.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117 Sci+Rep
2018 ; 8
(1
): 4131
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gab.com Text
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Accuracy of Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass
Spectrometry for Identification of Mycobacteria: a systematic review and
meta-analysis
#MMPMID29515167
Cao Y
; Wang L
; Ma P
; Fan W
; Gu B
; Ju S
Sci Rep
2018[Mar]; 8
(1
): 4131
PMID29515167
show ga
Mycobacterium species are a significant cause of morbidity and mortality
worldwide. The present study was carried out to systematically evaluate the
accuracy of Matrix-assisted laser desorption ionization-time of flight mass
spectroscopy (MALDI-TOF MS) for the identification of clinical pathogenic
mycobacteria. After a rigid selection process, 19 articles involving 2,593
mycobacteria isolates were included. The pooled result agreed with the reference
method identification for 85% of the isolates to genus level, with 71% (95% CI of
69% to 72%) correct to the species level. The MALDI-TOF MS correctly identified
92% of the M.tuberculosis isolates (95% CI of 0.87 to 0.96), and 68% of M.
bovisisolates (95% CI of 27% to 100%) to the species level. Mycobacterium
tuberculosis complex in solid media with reference strains using augmented
database showing more accurate identification. The identifying accuracy rate of
bioMérieuxVitek MS was slight higher than Bruker MALDI Biotyper (75% vs 72%).
However, opposite results were obtained in identifications of M. fortuitum, M.
kansasii, M. marinum, and M. terrae with these two systems. In summary, our
results demonstrate that application of MALDI-TOF MS in clinical pathogenic
mycobacteria identification is less satisfactory to date. Increasing need for
improvement is important especially at species level.