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10.1186/s12879-018-3015-y http://scihub22266oqcxt.onion/10.1186/s12879-018-3015-y C5840791!5840791 !29510659     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=29510659 &cmd=llinks): Failed to open stream: HTTP request failed! 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Next generation sequencing based pathogen analysis in a patient with neurocysticercosis: a case report |pdf=|usr=}}{{29510659 }} gab.com Text Next generation sequencing based pathogen analysis in a patient with neurocysticercosis: a case report JO: BMC Infect Dis http://www.kidney.de/pget.php?&tw=1&pmid=29510659 #FOAvip BACKGROUND: Accurate and early diagnosis of neurocysticercosis (NCC) remains a challenge due to the heterogeneity of its clinical, immunological and imaging characteristics. The presence of cysticercus DNA in cerebrospinal fluid (CSF) of NCC patients has been previously detected via conventional PCR assays. To the best of our knowledge, the use of CSF Next-Generation Sequencing (NGS) based pathogen analysis in patients with NCC infection has never been reported. CASE PRESENTATION: This study reports the clinical, imaging, and immunological features of a patient initially presenting with several months of headache who further developed a pure sensory stroke. NGS was used to detect the pathogen, and her CSF demonstrated the presence of Taenia solium-DNA. This finding was confirmed by a positive reaction to CSF cysticercosis antibodies. After antiparasitic treatment, secondary CSF NGS revealed the DNA index have dropped considerably compared to the initial NGS readings. CONCLUSIONS: NGS is a promising tool for the early and accurate diagnosis of central nervous system (CNS) infection, especially in the setting of atypical clinical manifestations. Further studies are required to evaluate the persistence of DNA in the CSF of patients. Twit Text FOAVip Next generation sequencing based pathogen analysis in a patient with neurocysticercosis: a case report ????BMC Infect Dis http://www.kidney.de/pget.php?&tw=1&pmid=29510659 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=29510659 #FOAvip English Wikipedia <ref>{{Cite pmid|29510659 }}</ref> Next generation sequencing based pathogen analysis in a patient with neurocysticercosis: a case report #MMPMID29510659 Liu P ; Weng X ; Zhou J ; Xu X ; He F ; Du Y ; Wu H ; Gong Y ; Peng G BMC Infect Dis 2018[Mar]; 18 (1 ): 113 PMID29510659 show ga BACKGROUND: Accurate and early diagnosis of neurocysticercosis (NCC) remains a challenge due to the heterogeneity of its clinical, immunological and imaging characteristics. The presence of cysticercus DNA in cerebrospinal fluid (CSF) of NCC patients has been previously detected via conventional PCR assays. To the best of our knowledge, the use of CSF Next-Generation Sequencing (NGS) based pathogen analysis in patients with NCC infection has never been reported. CASE PRESENTATION: This study reports the clinical, imaging, and immunological features of a patient initially presenting with several months of headache who further developed a pure sensory stroke. NGS was used to detect the pathogen, and her CSF demonstrated the presence of Taenia solium-DNA. This finding was confirmed by a positive reaction to CSF cysticercosis antibodies. After antiparasitic treatment, secondary CSF NGS revealed the DNA index have dropped considerably compared to the initial NGS readings. CONCLUSIONS: NGS is a promising tool for the early and accurate diagnosis of central nervous system (CNS) infection, especially in the setting of atypical clinical manifestations. Further studies are required to evaluate the persistence of DNA in the CSF of patients. |Adult [MESH] |Animals [MESH] |Anthelmintics/administration & dosage/therapeutic use [MESH] |Brain/diagnostic imaging [MESH] |DNA, Helminth/cerebrospinal fluid [MESH] |Female [MESH] |Headache/parasitology [MESH] |High-Throughput Nucleotide Sequencing/*methods [MESH] |Humans [MESH] |Magnetic Resonance Imaging [MESH] |Neurocysticercosis/*diagnosis/drug therapy/*etiology [MESH] |Praziquantel/administration & dosage/therapeutic use [MESH] |Stroke/etiology/parasitology [MESH]Next generation sequencing based pathogen analysis in a patient with (epmc).pdf DeepDyve Pubget Overpricing