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MicroRNA-183 promotes the proliferation and metastasis of renal cell carcinoma
through targeting Dickkopf-related protein 3
#MMPMID29552228
Zhang XL
; Xu G
; Zhou Y
; Yan JJ
Oncol Lett
2018[Apr]; 15
(4
): 6003-6008
PMID29552228
show ga
Renal cell carcinoma (RCC) is insensitive to conventional chemotherapy and
radiotherapy, and its prognosis remains poor. Targeted therapy is a novel
technique for the treatment of RCC. The present study aimed to determine the
precise effects of microRNA-183 (miR-183) on RCC. Reverse
transcription-quantitative polymerase chain reaction analysis was conducted in
order to analyze the expression level of miR-183 in RCC cell lines. Cell
transfection, viability assays and invasion assays were also performed, followed
by western blot analysis and a luciferase reporter assay, in order to examine the
effects of miR-183. The expression of miR-183 was upregulated in all four RCC
cell lines compared with the immortalized normal proximal tubule epithelial HK-2
cell line. The cell viability and invasion assays demonstrated that decreased
expression of miR-183 suppressed RCC cell proliferation and invasion in vitro.
Furthermore, the present study identified that Dickkopf-related protein-3 (DKK-3)
was a direct target of miR-183 in RCC. The results demonstrated that miR-183
functioned as an oncogene by downregulating DKK-3 expression and that it provided
a potential diagnostic, prognostic and therapeutic target for RCC.
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