Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=29545791
&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 500 Internal Server Error
in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215
Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\29545791
.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117 Front+Immunol
2018 ; 9
(ä): 324
Nephropedia Template TP
gab.com Text
Twit Text FOAVip
Twit Text #
English Wikipedia
Functional, Antigen-Specific Stem Cell Memory (T(SCM)) CD4(+) T Cells Are Induced
by Human Mycobacterium tuberculosis Infection
#MMPMID29545791
Mpande CAM
; Dintwe OB
; Musvosvi M
; Mabwe S
; Bilek N
; Hatherill M
; Nemes E
; Scriba TJ
Front Immunol
2018[]; 9
(ä): 324
PMID29545791
show ga
BACKGROUND: Maintenance of long-lasting immunity is thought to depend on stem
cell memory T cells (T(SCM)), which have superior self-renewing capacity,
longevity and proliferative potential compared with central memory (T(CM)) or
effector (T(EFF)) T cells. Our knowledge of T(SCM) derives primarily from studies
of virus-specific CD8(+) T(SCM). We aimed to determine if infection with
Mycobacterium tuberculosis (M. tb), the etiological agent of tuberculosis,
generates antigen-specific CD4(+) T(SCM) and to characterize their functional
ontology. METHODS: We studied T cell responses to natural M. tb infection in a
longitudinal adolescent cohort of recent QuantiFERON-TB Gold (QFT) converters and
three cross-sectional QFT(+) adult cohorts; and to bacillus Calmette-Guerin (BCG)
vaccination in infants. M. tb and/or BCG-specific CD4 T cells were detected by
flow cytometry using major histocompatibility complex class II tetramers bearing
Ag85, CFP-10, or ESAT-6 peptides, or by intracellular cytokine staining.
Transcriptomic analyses of M. tb-specific tetramer(+) CD4(+) T(SCM) (CD45RA(+)
CCR7(+) CD27(+)) were performed by microfluidic qRT-PCR, and functional and
phenotypic characteristics were confirmed by measuring expression of chemokine
receptors, cytotoxic molecules and cytokines using flow cytometry. RESULTS: M.
tb-specific T(SCM) were not detected in QFT-negative persons. After QFT
conversion frequencies of T(SCM) increased to measurable levels and remained
detectable thereafter, suggesting that primary M. tb infection induces T(SCM)
cells. Gene expression (GE) profiling of tetramer(+) T(SCM) showed that these
cells were distinct from bulk CD4(+) naïve T cells (T(N)) and shared features of
bulk T(SCM) and M. tb-specific tetramer(+) T(CM) and T(EFF) cells. These T(SCM)
were predominantly CD95(+) and CXCR3(+), markers typical of CD8(+) T(SCM).
Tetramer(+) T(SCM) expressed significantly higher protein levels of CCR5, CCR6,
CXCR3, granzyme A, granzyme K, and granulysin than bulk T(N) and T(SCM) cells. M.
tb-specific T(SCM) were also functional, producing IL-2, IFN-?, and TNF-? upon
antigen stimulation, and their frequencies correlated positively with long-term
BCG-specific CD4(+) T cell proliferative potential after infant vaccination.
CONCLUSION: Human infection with M. tb induced distinct, antigen-specific CD4(+)
T(SCM) cells endowed with effector functions, including expression of cytotoxic
molecules and Th1 cytokines, and displayed chemokine receptor profiles consistent
with memory Th1/17 cells. Induction of CD4(+) T(SCM) should be considered for
vaccination approaches that aim to generate long-lived memory T cells against M.
tb.
free
free
free
