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10.1002/cre2.17 http://scihub22266oqcxt.onion/10.1002/cre2.17 C5839176!5839176!29744147     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Clin+Exp+Dent+Res 2016 ; 2 (1): 35-43 Nephropedia Template TP {{tp|p=29744147|t=2016. Relative effectiveness of azithromycin in killing intracellular Porphyromonas gingivalis |pdf=|usr=}}{{29744147}} gab.com Text Relative effectiveness of azithromycin in killing intracellular Porphyromonas gingivalis JO: Clin Exp Dent Res http://www.kidney.de/pget.php?&tw=1&pmid=29744147 #FOAvip Invasive infections by Porphyromonas gingivalis are associated with persistent periodontal attachment loss and can be difficult to eliminate by scaling and root planing. Azithromycin (AZM) inhibits P.?gingivalis and is actively accumulated by most human cells. We used an in vitro infection model to compare the effectiveness of AZM in killing intracellular P.?gingivalis to the combined regimen of amoxicillin (AMX) and metronidazole (MET). Transport of [3H]?AZM by human gingival fibroblasts was characterized. Monolayers of Smulow?Glickman gingival epithelial cells or gingival fibroblasts were infected with P.?gingivalis (strain 33277 or W83). After extracellular bacteria were eliminated with teicoplanin, infected cells were treated with therapeutic concentrations of AZM, AMX, or AMX?+?MET. Viable intracellular bacteria were released by cell lysis and plated on blood agar for enumeration. Antimicrobial activity against planktonic P.?gingivalis was also evaluated. While survival of intraepithelial P.?gingivalis 33277 was not significantly different after treatment with the three regimens, survival in infected fibroblasts was significantly lower after AZM treatment (65.9?±?5.5%) compared with AMX (92.2?±?3.5%) or AMX?+?MET (79.8?±?5.2%, P?<?0.01). Carnitine, a competitive inhibitor of AZM transport, reduced killing by AZM by ~55% (P?<?0.05). Survival of intrafibroblast P.?gingivalis W83 was also significantly lower after AZM treatment compared with the other regimens (P?<?0.05). At therapeutic concentrations, AZM was significantly more active against intracellular P.?gingivalis than against planktonic P.?gingivalis (P?<?0.0083). Gingival epithelial cells and fibroblasts possess a transport system that accumulates AZM and enhances elimination of intracellular P.?gingivalis. Compared with the combination of AMX and MET, AZM was equally effective against intraepithelial P.?gingivalis 33277 and significantly more effective against both strains of P.?gingivalis from infected gingival fibroblasts. The results suggest that AZM could be a reasonable alternative to the regimen of AMX and MET for periodontal patients who should not take these agents due to known side effects or compliance issues. Twit Text FOAVip Relative effectiveness of azithromycin in killing intracellular Porphyromonas gingivalis ????Clin Exp Dent Res http://www.kidney.de/pget.php?&tw=1&pmid=29744147 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=29744147 #FOAvip English Wikipedia <ref>{{Cite pmid|29744147}}</ref> Relative effectiveness of azithromycin in killing intracellular Porphyromonas gingivalis #MMPMID29744147 Lai P; Walters JD Clin Exp Dent Res 2016[Jun]; 2 (1): 35-43 PMID29744147show ga Invasive infections by Porphyromonas gingivalis are associated with persistent periodontal attachment loss and can be difficult to eliminate by scaling and root planing. Azithromycin (AZM) inhibits P.?gingivalis and is actively accumulated by most human cells. We used an in vitro infection model to compare the effectiveness of AZM in killing intracellular P.?gingivalis to the combined regimen of amoxicillin (AMX) and metronidazole (MET). Transport of [3H]?AZM by human gingival fibroblasts was characterized. Monolayers of Smulow?Glickman gingival epithelial cells or gingival fibroblasts were infected with P.?gingivalis (strain 33277 or W83). After extracellular bacteria were eliminated with teicoplanin, infected cells were treated with therapeutic concentrations of AZM, AMX, or AMX?+?MET. Viable intracellular bacteria were released by cell lysis and plated on blood agar for enumeration. Antimicrobial activity against planktonic P.?gingivalis was also evaluated. While survival of intraepithelial P.?gingivalis 33277 was not significantly different after treatment with the three regimens, survival in infected fibroblasts was significantly lower after AZM treatment (65.9?±?5.5%) compared with AMX (92.2?±?3.5%) or AMX?+?MET (79.8?±?5.2%, P? äRelative effectiveness of azithromycin in killing intracellular Porphy(epmc).pdf DeepDyve Pubget Overpricing