Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=29510727
&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests
in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215
Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 253.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 253.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\29510727
.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117 J+Exp+Clin+Cancer+Res
2018 ; 37
(1
): 50
Nephropedia Template TP
gab.com Text
Twit Text FOAVip
Twit Text #
English Wikipedia
EEF1D overexpression promotes osteosarcoma cell proliferation by facilitating
Akt-mTOR and Akt-bad signaling
#MMPMID29510727
Cheng DD
; Li SJ
; Zhu B
; Zhou SM
; Yang QC
J Exp Clin Cancer Res
2018[Mar]; 37
(1
): 50
PMID29510727
show ga
BACKGROUND: Dysregulation of eukaryotic translation elongation factor 1 delta
(EEF1D) in cancers has been reported; however, the role and mechanisms of EEF1D
in osteosarcoma remain poorly understood. The aim of this study is to investigate
the expression and role of EEF1D in osteosarcoma and to elucidate its underlying
mechanisms. METHODS: The expression of EEF1D in osteosarcomas and cell lines was
evaluated by qRT-PCR, Western blotting and immunohistochemistry. EEF1D knockdown
using small interfering RNA (siRNA) was employed to analyze the role of EEF1D in
osteosarcoma cell proliferation and cell cycle progression. The host signaling
pathways affected by EEF1D knockdown were detected using PathScanŽ intracellular
signaling array kit. RESULTS: The expression of EEF1D was found to be
up-regulated in human osteosarcoma tissues and cell lines. Its expression was
positively correlated with Enneking stage and the tumor recurrence. EEF1D
knockdown inhibited osteosarcoma cell proliferation, colony-forming ability, and
cell cycle G2/M transition in vitro. In addition, EEF1D knockdown decreased the
levels of phospho-Akt, phospho-mTOR, and phospho-Bad proteins. CONCLUSIONS: EEF1D
is upregulated in osteosarcoma and plays a tumor promoting role by facilitating
Akt-mTOR and Akt-Bad signaling pathways. Accordingly, EEF1D is a potential target
for cancer therapy.
free
free
free
