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10.1186/s12864-018-4555-7 http://scihub22266oqcxt.onion/10.1186/s12864-018-4555-7 C5833154!5833154!29495964     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       BMC+Genomics 2018 ; 19 (ä): ä Nephropedia Template TP {{tp|p=29495964|t=2018. Improving eukaryotic genome annotation using single molecule mRNA sequencing |pdf=|usr=}}{{29495964}} gab.com Text Improving eukaryotic genome annotation using single molecule mRNA sequencing JO: BMC Genomics http://www.kidney.de/pget.php?&tw=1&pmid=29495964 #FOAvip Background: The advantages of Pacific Biosciences (PacBio) single-molecule real-time (SMRT) technology include long reads, low systematic bias, and high consensus read accuracy. Here we use these attributes to improve on the genome annotation of the parasitic hookworm Ancylostoma ceylanicum using PacBio RNA-Seq. Results: We sequenced 192,888 circular consensus sequences (CCS) derived from cDNAs generated using the CloneTech SMARTer system. These SMARTer-SMRT libraries were normalized and size-selected providing a robust population of expressed structural genes for subsequent genome annotation. We demonstrate PacBio mRNA sequences based genome annotation improvement, compared to genome annotation using conventional sequencing-by-synthesis alone, by identifying 1609 (9.2%) new genes, extended the length of 3965 (26.7%) genes and increased the total genomic exon length by 1.9 Mb (12.4%). Non-coding sequence representation (primarily from UTRs based on dT reverse transcription priming) was particularly improved, increasing in total length by fifteen-fold, by increasing both the length and number of UTR exons. In addition, the UTR data provided by these CCS allowed for the identification of a novel SL2 splice leader sequence for A. ceylanicum and an increase in the number and proportion of functionally annotated genes. RNA-seq data also confirmed some of the newly annotated genes and gene features. Conclusion: Overall, PacBio data has supported a significant improvement in gene annotation in this genome, and is an appealing alternative or complementary technique for genome annotation to the other transcript sequencing technologies. Electronic supplementary material: The online version of this article (10.1186/s12864-018-4555-7) contains supplementary material, which is available to authorized users. Twit Text FOAVip Improving eukaryotic genome annotation using single molecule mRNA sequencing ????BMC Genomics http://www.kidney.de/pget.php?&tw=1&pmid=29495964 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=29495964 #FOAvip English Wikipedia <ref>{{Cite pmid|29495964}}</ref> Improving eukaryotic genome annotation using single molecule mRNA sequencing #MMPMID29495964 Magrini V; Gao X; Rosa BA; McGrath S; Zhang X; Hallsworth-Pepin K; Martin J; Hawdon J; Wilson RK; Mitreva M BMC Genomics 2018[]; 19 (ä): ä PMID29495964show ga Background: The advantages of Pacific Biosciences (PacBio) single-molecule real-time (SMRT) technology include long reads, low systematic bias, and high consensus read accuracy. Here we use these attributes to improve on the genome annotation of the parasitic hookworm Ancylostoma ceylanicum using PacBio RNA-Seq. Results: We sequenced 192,888 circular consensus sequences (CCS) derived from cDNAs generated using the CloneTech SMARTer system. These SMARTer-SMRT libraries were normalized and size-selected providing a robust population of expressed structural genes for subsequent genome annotation. We demonstrate PacBio mRNA sequences based genome annotation improvement, compared to genome annotation using conventional sequencing-by-synthesis alone, by identifying 1609 (9.2%) new genes, extended the length of 3965 (26.7%) genes and increased the total genomic exon length by 1.9 Mb (12.4%). Non-coding sequence representation (primarily from UTRs based on dT reverse transcription priming) was particularly improved, increasing in total length by fifteen-fold, by increasing both the length and number of UTR exons. In addition, the UTR data provided by these CCS allowed for the identification of a novel SL2 splice leader sequence for A. ceylanicum and an increase in the number and proportion of functionally annotated genes. RNA-seq data also confirmed some of the newly annotated genes and gene features. Conclusion: Overall, PacBio data has supported a significant improvement in gene annotation in this genome, and is an appealing alternative or complementary technique for genome annotation to the other transcript sequencing technologies. Electronic supplementary material: The online version of this article (10.1186/s12864-018-4555-7) contains supplementary material, which is available to authorized users. äImproving eukaryotic genome annotation using single molecule mRNA sequ(epmc).pdf DeepDyve Pubget Overpricing