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10.1038/nmeth.4582 http://scihub22266oqcxt.onion/10.1038/nmeth.4582 C5831505!5831505!29355846     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nat+Methods 2018 ; 15 (3): 221-5 Nephropedia Template TP {{tp|p=29355846|t=2018. TimeLapse-seq: Adding a temporal dimension to RNA sequencing through nucleoside recoding |pdf=|usr=}}{{29355846}} gab.com Text TimeLapse-seq: Adding a temporal dimension to RNA sequencing through nucleoside recoding JO: Nat Methods http://www.kidney.de/pget.php?&tw=1&pmid=29355846 #FOAvip RNA sequencing (RNA-seq) offers a snapshot of cellular RNA populations, but not temporal information about the sequenced RNA. Here we report TimeLapse-seq, a chemical method that uses oxidative-nucleophilic-aromatic-substitution to convert 4-thiouridine into cytidine analogues, yielding apparent U-to-C mutations that mark new transcripts upon sequencing. TimeLapse-seq is a single molecule approach that is adaptable to many applications, and reveals RNA dynamics and induced differential expression concealed in traditional RNA-seq. Twit Text FOAVip TimeLapse-seq: Adding a temporal dimension to RNA sequencing through nucleoside recoding ????Nat Methods http://www.kidney.de/pget.php?&tw=1&pmid=29355846 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=29355846 #FOAvip English Wikipedia <ref>{{Cite pmid|29355846}}</ref> TimeLapse-seq: Adding a temporal dimension to RNA sequencing through nucleoside recoding #MMPMID29355846 Schofield JA; Duffy EE; Kiefer L; Sullivan MC; Simon MD Nat Methods 2018[Mar]; 15 (3): 221-5 PMID29355846show ga RNA sequencing (RNA-seq) offers a snapshot of cellular RNA populations, but not temporal information about the sequenced RNA. Here we report TimeLapse-seq, a chemical method that uses oxidative-nucleophilic-aromatic-substitution to convert 4-thiouridine into cytidine analogues, yielding apparent U-to-C mutations that mark new transcripts upon sequencing. TimeLapse-seq is a single molecule approach that is adaptable to many applications, and reveals RNA dynamics and induced differential expression concealed in traditional RNA-seq. äTimeLapse-seq: Adding a temporal dimension to RNA sequencing through n(epmc).pdf DeepDyve Pubget Overpricing