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Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Stem+Cell+Reports 2018 ; 10 (2): 627-41 Nephropedia Template TP
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Compartmentalization of HP1 Proteins in Pluripotency Acquisition and Maintenance #MMPMID29358085
Zaidan NZ; Walker KJ; Brown JE; Schaffer LV; Scalf M; Shortreed MR; Iyer G; Smith LM; Sridharan R
Stem Cell Reports 2018[Feb]; 10 (2): 627-41 PMID29358085show ga
The heterochromatin protein 1 (HP1) family is involved in various functions with maintenance of chromatin structure. During murine somatic cell reprogramming, we find that early depletion of HP1? reduces the generation of induced pluripotent stem cells, while late depletion enhances the process, with a concomitant change from a centromeric to nucleoplasmic localization and elongation-associated histone H3.3 enrichment. Depletion of heterochromatin anchoring protein SENP7 increased reprogramming efficiency to a similar extent as HP1?, indicating the importance of HP1? release from chromatin for pluripotency acquisition. HP1? interacted with OCT4 and DPPA4 in HP1? and HP1? knockouts and in H3K9 methylation depleted H3K9M embryonic stem cell (ESC) lines. HP1? and HP1? complexes in ESCs differed in association with histones, the histone chaperone CAF1 complex, and specific components of chromatin-modifying complexes such as DPY30, implying distinct functional contributions. Taken together, our results reveal the complex contribution of the HP1 proteins to pluripotency.