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10.1080/19420862.2017.1406570 http://scihub22266oqcxt.onion/10.1080/19420862.2017.1406570 C5825193!5825193 !29227213     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=29227213 &cmd=llinks): Failed to open stream: HTTP request failed! 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Productive common light chain libraries yield diverse panels of high affinity bispecific antibodies |pdf=|usr=}}{{29227213 }} gab.com Text Productive common light chain libraries yield diverse panels of high affinity bispecific antibodies JO: MAbs http://www.kidney.de/pget.php?&tw=1&pmid=29227213 #FOAvip The commercial success of bispecific antibodies generally has been hindered by the complexities associated with generating appropriate molecules for both research scale and large scale manufacturing purposes. Bispecific IgG (BsIgG) based on two antibodies that use an identical common light chain can be combined with a minimal set of Fc mutations to drive heavy chain heterodimerization in order to address these challenges. However, the facile generation of common light chain antibodies with properties similar to traditional monoclonal antibodies has not been demonstrated and they have only been used sparingly. Here, we describe the design of a synthetic human antibody library based on common light chains to generate antibodies with biochemical and biophysical properties that are indistinguishable to traditional therapeutic monoclonal antibodies. We used this library to generate diverse panels of well-behaved, high affinity antibodies toward a variety of epitopes across multiple antigens, including mouse 4-1BB, a therapeutically important T cell costimulatory receptor. Over 200 BsIgG toward 4-1BB were generated using an automated purification method we developed that enables milligram-scale production of BsIgG. This approach allowed us to identify antibodies with a wide range of agonistic activity that are being used to further investigate the therapeutic potential of antibodies targeting one or more epitopes of 4-1BB. Twit Text FOAVip Productive common light chain libraries yield diverse panels of high affinity bispecific antibodies ????MAbs http://www.kidney.de/pget.php?&tw=1&pmid=29227213 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=29227213 #FOAvip English Wikipedia <ref>{{Cite pmid|29227213 }}</ref> Productive common light chain libraries yield diverse panels of high affinity bispecific antibodies #MMPMID29227213 Van Blarcom T ; Lindquist K ; Melton Z ; Cheung WL ; Wagstrom C ; McDonough D ; Valle Oseguera C ; Ding S ; Rossi A ; Potluri S ; Sundar P ; Pitts S ; Sirota M ; Galindo Casas M ; Yan Y ; Jones J ; Roe-Zurz Z ; Srivatsa Srinivasan S ; Zhai W ; Pons J ; Rajpal A ; Chaparro-Riggers J MAbs 2018[Feb]; 10 (2 ): 256-268 PMID29227213 show ga The commercial success of bispecific antibodies generally has been hindered by the complexities associated with generating appropriate molecules for both research scale and large scale manufacturing purposes. Bispecific IgG (BsIgG) based on two antibodies that use an identical common light chain can be combined with a minimal set of Fc mutations to drive heavy chain heterodimerization in order to address these challenges. However, the facile generation of common light chain antibodies with properties similar to traditional monoclonal antibodies has not been demonstrated and they have only been used sparingly. Here, we describe the design of a synthetic human antibody library based on common light chains to generate antibodies with biochemical and biophysical properties that are indistinguishable to traditional therapeutic monoclonal antibodies. We used this library to generate diverse panels of well-behaved, high affinity antibodies toward a variety of epitopes across multiple antigens, including mouse 4-1BB, a therapeutically important T cell costimulatory receptor. Over 200 BsIgG toward 4-1BB were generated using an automated purification method we developed that enables milligram-scale production of BsIgG. This approach allowed us to identify antibodies with a wide range of agonistic activity that are being used to further investigate the therapeutic potential of antibodies targeting one or more epitopes of 4-1BB. |*Antibodies, Bispecific [MESH] |*Immunoglobulin Light Chains [MESH] |*Peptide Library [MESH] |Animals [MESH] |Humans [MESH] |Mice [MESH]Productive common light chain libraries yield diverse panels of high a(epmc).pdf DeepDyve Pubget Overpricing