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10.1038/s41598-018-21004-w http://scihub22266oqcxt.onion/10.1038/s41598-018-21004-w C5813043!5813043 !29445164     free     free     free Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 261.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 261.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 261.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 261.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 261.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\29445164 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Sci+Rep 2018 ; 8 (1 ): 3023 Nephropedia Template TP {{tp|p=29445164 |t=2018. Inactivation of TRPM7 kinase in mice results in enlarged spleens, reduced T-cell proliferation and diminished store-operated calcium entry |pdf=|usr=}}{{29445164 }} gab.com Text Inactivation of TRPM7 kinase in mice results in enlarged spleens, reduced T-cell proliferation and diminished store-operated calcium entry JO: Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=29445164 #FOAvip T lymphocytes enlarge (blast) and proliferate in response to antigens in a multistep program that involves obligatory cytosolic calcium elevations. Store-operated calcium entry (SOCE) pathway is the primary source of Ca(2+) in these cells. Here, we describe a novel modulator of blastogenesis, proliferation and SOCE: the TRPM7 channel kinase. TRPM7 kinase-dead (KD) K1646R knock-in mice exhibited splenomegaly and impaired blastogenic responses elicited by PMA/ionomycin or anti-CD3/CD28 antibodies. Splenic T-cell proliferation in vitro was weaker in the mutant compared to wildtype littermates. TRPM7 current magnitudes in WT and KD mouse T cells were, however, similar. We tested the dependence of T-cell proliferation on external Ca(2+) and Mg(2+) concentrations. At a fixed [Mg(2+)(o)] of ~0.4?mM, Ca(2+)(o) stimulated proliferation with a steep concentration dependence and vice versa, at a fixed [Ca(2+)(o)] of ~0.4?mM, Mg(2+)(o) positively regulated proliferation but with a shallower dependence. Proliferation was significantly lower in KD mouse than in wildtype at all Ca(2+) and Mg(2+) concentrations. Ca(2+) elevations elicited by anti-CD3 antibody were diminished in KD mutant T cells and SOCE measured in activated KD splenocytes was reduced. These results demonstrate that a functional TRPM7 kinase supports robust SOCE, blastogenesis and proliferation, whereas its inactivation suppresses these cellular events. Twit Text FOAVip Inactivation of TRPM7 kinase in mice results in enlarged spleens, reduced T-cell proliferation and diminished store-operated calcium entry ????Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=29445164 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=29445164 #FOAvip English Wikipedia <ref>{{Cite pmid|29445164 }}</ref> Inactivation of TRPM7 kinase in mice results in enlarged spleens, reduced T-cell proliferation and diminished store-operated calcium entry #MMPMID29445164 Beesetty P ; Wieczerzak KB ; Gibson JN ; Kaitsuka T ; Luu CT ; Matsushita M ; Kozak JA Sci Rep 2018[Feb]; 8 (1 ): 3023 PMID29445164 show ga T lymphocytes enlarge (blast) and proliferate in response to antigens in a multistep program that involves obligatory cytosolic calcium elevations. Store-operated calcium entry (SOCE) pathway is the primary source of Ca(2+) in these cells. Here, we describe a novel modulator of blastogenesis, proliferation and SOCE: the TRPM7 channel kinase. TRPM7 kinase-dead (KD) K1646R knock-in mice exhibited splenomegaly and impaired blastogenic responses elicited by PMA/ionomycin or anti-CD3/CD28 antibodies. Splenic T-cell proliferation in vitro was weaker in the mutant compared to wildtype littermates. TRPM7 current magnitudes in WT and KD mouse T cells were, however, similar. We tested the dependence of T-cell proliferation on external Ca(2+) and Mg(2+) concentrations. At a fixed [Mg(2+)(o)] of ~0.4?mM, Ca(2+)(o) stimulated proliferation with a steep concentration dependence and vice versa, at a fixed [Ca(2+)(o)] of ~0.4?mM, Mg(2+)(o) positively regulated proliferation but with a shallower dependence. Proliferation was significantly lower in KD mouse than in wildtype at all Ca(2+) and Mg(2+) concentrations. Ca(2+) elevations elicited by anti-CD3 antibody were diminished in KD mutant T cells and SOCE measured in activated KD splenocytes was reduced. These results demonstrate that a functional TRPM7 kinase supports robust SOCE, blastogenesis and proliferation, whereas its inactivation suppresses these cellular events. |Animals [MESH] |Calcium Channels/metabolism [MESH] |Calcium Signaling/physiology [MESH] |Calcium/*metabolism [MESH] |Cell Proliferation/physiology [MESH] |Gene Knock-In Techniques/methods [MESH] |Lymphocyte Activation/physiology [MESH] |Magnesium/metabolism [MESH] |Mice [MESH] |Mice, Inbred C57BL [MESH] |Spleen/pathology [MESH] |Splenomegaly/metabolism [MESH] |Stromal Interaction Molecule 1/metabolism [MESH] |T-Lymphocytes/metabolism/physiology [MESH]Inactivation of TRPM7 kinase in mice results in enlarged spleens, redu(epmc).pdf DeepDyve Pubget Overpricing