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10.1038/s41551-017-0152-3 http://scihub22266oqcxt.onion/10.1038/s41551-017-0152-3 C5811202!5811202 !29456878     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=29456878 &cmd=llinks): Failed to open stream: HTTP request failed! 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Non-destructive two-photon excited fluorescence imaging identifies early nodules in calcific aortic-valve disease |pdf=|usr=}}{{29456878 }} gab.com Text Non-destructive two-photon excited fluorescence imaging identifies early nodules in calcific aortic-valve disease JO: Nat Biomed Eng http://www.kidney.de/pget.php?&tw=1&pmid=29456878 #FOAvip Calcifications occur during the development of healthy bone, and at the onset of calcific aortic-valve disease (CAVD) and many other pathologies. Although the mechanisms regulating early calcium deposition are not fully understood, they may provide targets for new treatments and for early interventions. Here, we show that two-photon excited fluorescence (TPEF) can provide quantitative and sensitive readouts of calcific nodule formation, in particular in the context of CAVD. Specifically, by means of the decomposition of TPEF spectral images from excised human CAVD valves and from rat bone prior to and following demineralization, as well as from calcific nodules formed within engineered gels, we identified an endogenous fluorophore that correlates with the level of mineralization in the samples. We then developed a ratiometric imaging approach that provides a quantitative readout of the presence of mineral deposits in early calcifications. TPEF should enable non-destructive, high-resolution imaging of three-dimensional tissue specimens for the assessment of the presence of calcification. Twit Text FOAVip Non-destructive two-photon excited fluorescence imaging identifies early nodules in calcific aortic-valve disease ????Nat Biomed Eng http://www.kidney.de/pget.php?&tw=1&pmid=29456878 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=29456878 #FOAvip English Wikipedia <ref>{{Cite pmid|29456878 }}</ref> Non-destructive two-photon excited fluorescence imaging identifies early nodules in calcific aortic-valve disease #MMPMID29456878 Baugh LM ; Liu Z ; Quinn KP ; Osseiran S ; Evans CL ; Huggins GS ; Hinds PW ; Black LD 3rd ; Georgakoudi I Nat Biomed Eng 2017[Nov]; 1 (11 ): 914-924 PMID29456878 show ga Calcifications occur during the development of healthy bone, and at the onset of calcific aortic-valve disease (CAVD) and many other pathologies. Although the mechanisms regulating early calcium deposition are not fully understood, they may provide targets for new treatments and for early interventions. Here, we show that two-photon excited fluorescence (TPEF) can provide quantitative and sensitive readouts of calcific nodule formation, in particular in the context of CAVD. Specifically, by means of the decomposition of TPEF spectral images from excised human CAVD valves and from rat bone prior to and following demineralization, as well as from calcific nodules formed within engineered gels, we identified an endogenous fluorophore that correlates with the level of mineralization in the samples. We then developed a ratiometric imaging approach that provides a quantitative readout of the presence of mineral deposits in early calcifications. TPEF should enable non-destructive, high-resolution imaging of three-dimensional tissue specimens for the assessment of the presence of calcification. äNon-destructive two-photon excited fluorescence imaging identifies ear(epmc).pdf DeepDyve Pubget Overpricing