Anal Biochem 2018[Jul]; 552 (ä): 50-9 PMID28711444show ga
The mitochondrial membrane potential (??m) generated by proton pumps (Complexes I, III and IV) is an essential component in the process of energy storage during oxidative phosphorylation. Together with the proton gradient (?pH), ??m forms the transmembrane potential of hydrogen ions which is harnessed to make ATP. The levels of ??m and ATP in the cell are kept relatively stable although there are limited fluctuations of both these factors that can occur reflecting normal physiological activity. However, sustained changes in both factors may be deleterious. A long-lasting drop or rise of ??mvs normal levels may induce unwanted loss of cell viability and be a cause of various pathologies. Among other factors, ??m plays a key role in mitochondrial homeostasis through selective elimination of dysfunctional mitochondria. It is also a driving force for transport of ions (other than H+) and proteins which are necessary for healthy mitochondrial functioning. We propose additional potential mechanisms for which ??m is essential for maintenance of cellular health and viability and provide recommendations how to accurately measure ??m in a cell and discuss potential sources of artifacts.
free
free
free
Anal+Biochem 2018 ; 552 (ä): 50-9
