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10.1073/pnas.1714658115

http://scihub22266oqcxt.onion/10.1073/pnas.1714658115
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suck abstract from ncbi


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pmid29298913      Proc+Natl+Acad+Sci+U+S+A 2018 ; 115 (3): 567-72
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  • Cross-genus rebooting of custom-made, synthetic bacteriophage genomes in L-form bacteria #MMPMID29298913
  • Kilcher S; Studer P; Muessner C; Klumpp J; Loessner MJ
  • Proc Natl Acad Sci U S A 2018[Jan]; 115 (3): 567-72 PMID29298913show ga
  • The unique host specificity and antimicrobial activity of bacterial viruses have inspired many diagnostic and antibacterial applications in industry, agriculture, and medicine. Because of the rise in antibiotic-resistant infections, phage therapy is a reemerging field of interest. Many restrictions that are associated with the use of native, isolated phages can be overcome by genetic engineering. Thus, efficient genome-editing tools are needed to unleash the full potential of phage therapy and biotechnology. In vitro assembly of synthetic virus genomes and the rapid isolation of corresponding phages is an important step in this direction. By using L-form bacteria as rebooting compartments of synthetic genomes, we report a simple, highly efficient, and broadly applicable technology that enables engineering of diverse phage families.
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