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10.4172/2324-8955.1000178 http://scihub22266oqcxt.onion/10.4172/2324-8955.1000178 C5770218!5770218!29349092     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Virol+Antivir+Res 2018 ; 7 (1): 1-20 Nephropedia Template TP {{tp|p=29349092|t=2018. Cathepsin B plays a key role in optimal production of the influenza A virus |pdf=|usr=}}{{29349092}} gab.com Text Cathepsin B plays a key role in optimal production of the influenza A virus JO: J Virol Antivir Res http://www.kidney.de/pget.php?&tw=1&pmid=29349092 #FOAvip Background: Influenza A virus (IAV) is the etiologic agent of the febrile respiratory illness, commonly referred to as ?flu?. The lysosomal protease cathepsin B (CTSB) has shown to be involved in the lifecycle of various viruses. Here, we examined the role of CTSB in the IAV lifecycle. Methods: CTSB-deficient (CTSB?/?) macrophages and the human lung epithelial cell line A549 cells treated with CA-074Me were infected with the A/Puerto Rico/8/34 strain of IAV (IAV-PR8). Viral entry and propagation were measured through quantitative real-time RT-PCR; production and localization of hemagglutinin (HA) protein in the infected host cells were analysed by Western blots, flow cytometry and confocal microscopy; production of progeny viruses were measured by a hemagglutination assay. Results: CTSB?/? macrophages and CA-074Me-treated A549 cells had no defects in incorporating IAV-PR8 virions and permitting viral RNA synthesis. However, these cells produced significantly lower amounts of HA protein and progeny virions than wild-type or untreated cells. Conclusion: These data indicate that CTSB is involved in the expression of IAV-PR8 HA protein and subsequent optimal production of IAV-PR8 progeny virions. Targeting CTSB can be a novel therapeutic strategy for treating IAV infection. Twit Text FOAVip Cathepsin B plays a key role in optimal production of the influenza A virus ????J Virol Antivir Res http://www.kidney.de/pget.php?&tw=1&pmid=29349092 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=29349092 #FOAvip English Wikipedia <ref>{{Cite pmid|29349092}}</ref> Cathepsin B plays a key role in optimal production of the influenza A virus #MMPMID29349092 Coleman MD; Ha SD; Haeryfar SM; Barr SD; Kim SO J Virol Antivir Res 2018[Apr]; 7 (1): 1-20 PMID29349092show ga Background: Influenza A virus (IAV) is the etiologic agent of the febrile respiratory illness, commonly referred to as ?flu?. The lysosomal protease cathepsin B (CTSB) has shown to be involved in the lifecycle of various viruses. Here, we examined the role of CTSB in the IAV lifecycle. Methods: CTSB-deficient (CTSB?/?) macrophages and the human lung epithelial cell line A549 cells treated with CA-074Me were infected with the A/Puerto Rico/8/34 strain of IAV (IAV-PR8). Viral entry and propagation were measured through quantitative real-time RT-PCR; production and localization of hemagglutinin (HA) protein in the infected host cells were analysed by Western blots, flow cytometry and confocal microscopy; production of progeny viruses were measured by a hemagglutination assay. Results: CTSB?/? macrophages and CA-074Me-treated A549 cells had no defects in incorporating IAV-PR8 virions and permitting viral RNA synthesis. However, these cells produced significantly lower amounts of HA protein and progeny virions than wild-type or untreated cells. Conclusion: These data indicate that CTSB is involved in the expression of IAV-PR8 HA protein and subsequent optimal production of IAV-PR8 progeny virions. Targeting CTSB can be a novel therapeutic strategy for treating IAV infection. äCathepsin B plays a key role in optimal production of the influenza A (epmc).pdf DeepDyve Pubget Overpricing