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10.7326/M17-0085 http://scihub22266oqcxt.onion/10.7326/M17-0085 C5721525!5721525!28586923     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Ann+Intern+Med 2017 ; 167 (1): 1-7 Nephropedia Template TP {{tp|p=28586923|t=2017. Presence of Human Hepegivirus-1 in a Cohort of People Who Inject Drugs |pdf=|usr=}}{{28586923}} gab.com Text Presence of Human Hepegivirus-1 in a Cohort of People Who Inject Drugs JO: Ann Intern Med http://www.kidney.de/pget.php?&tw=1&pmid=28586923 #FOAvip Background: Next-generation metagenomic sequencing (NGMS) has opened new frontiers in microbial discovery but has been clinically characterized in only a few settings. Objective: To explore the plasma virome of persons who inject drugs while characterizing the sensitivity and accuracy of NGMS compared to quantitative clinical standards. Design: Longitudinal and cross-sectional studies. Participants: Hepatitis C virus (HCV) and human immunodeficiency virus (HIV) co-infected persons enrolled in a clinical trial (NCT01285050) and/or a well-characterized cohort study of persons who have injected drugs. Measurements: Viral nucleic acid in plasma by NGMS and quantitative polymerase chain reaction (PCR). Results: NGMS generated a total of 600 million reads, which included the expected HIV and HCV RNA sequences. HIV and HCV reads were consistently identified only when samples contained >10,000 copies or IU/ml as determined by quantitative PCR. A novel RNA virus, human hepegivirus-1 (HHpgV-1) was also detected by NGMS in 4 samples from 2 persons in the clinical trial. Because of this unexpected finding, using a quantitative PCR assay for HHpgV-1, infection was also detected in 17 (10.9%) of 156 members of a cohort of persons who injected drugs in whom HHpgV-1 viremia persisted for a median of at least 4538 days and was associated with detection of other bloodborne viruses such as HCV RNA and SEN virus D Limitations: The medical importance of HHpgV-1infection remains unknown. Conclusions: Although NGMS is insensitive to detection of viruses with relatively low plasma nucleic acid concentrations, it may have a broad potential for discovery of new viral infections of potential medical importance like HHpgV-1. Twit Text FOAVip Presence of Human Hepegivirus-1 in a Cohort of People Who Inject Drugs ????Ann Intern Med http://www.kidney.de/pget.php?&tw=1&pmid=28586923 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28586923 #FOAvip English Wikipedia <ref>{{Cite pmid|28586923}}</ref> Presence of Human Hepegivirus-1 in a Cohort of People Who Inject Drugs #MMPMID28586923 Kandathil AJ; Breitwieser FP; Sachithanandham J; Robinson M; Mehta SH; Timp W; Salzberg SL; Thomas DL; Balagopal A Ann Intern Med 2017[Jul]; 167 (1): 1-7 PMID28586923show ga Background: Next-generation metagenomic sequencing (NGMS) has opened new frontiers in microbial discovery but has been clinically characterized in only a few settings. Objective: To explore the plasma virome of persons who inject drugs while characterizing the sensitivity and accuracy of NGMS compared to quantitative clinical standards. Design: Longitudinal and cross-sectional studies. Participants: Hepatitis C virus (HCV) and human immunodeficiency virus (HIV) co-infected persons enrolled in a clinical trial (NCT01285050) and/or a well-characterized cohort study of persons who have injected drugs. Measurements: Viral nucleic acid in plasma by NGMS and quantitative polymerase chain reaction (PCR). Results: NGMS generated a total of 600 million reads, which included the expected HIV and HCV RNA sequences. HIV and HCV reads were consistently identified only when samples contained >10,000 copies or IU/ml as determined by quantitative PCR. A novel RNA virus, human hepegivirus-1 (HHpgV-1) was also detected by NGMS in 4 samples from 2 persons in the clinical trial. Because of this unexpected finding, using a quantitative PCR assay for HHpgV-1, infection was also detected in 17 (10.9%) of 156 members of a cohort of persons who injected drugs in whom HHpgV-1 viremia persisted for a median of at least 4538 days and was associated with detection of other bloodborne viruses such as HCV RNA and SEN virus D Limitations: The medical importance of HHpgV-1infection remains unknown. Conclusions: Although NGMS is insensitive to detection of viruses with relatively low plasma nucleic acid concentrations, it may have a broad potential for discovery of new viral infections of potential medical importance like HHpgV-1. äPresence of Human Hepegivirus-1 in a Cohort of People Who Inject Drugs(epmc).pdf DeepDyve Pubget Overpricing