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10.1007/s11302-017-9588-x http://scihub22266oqcxt.onion/10.1007/s11302-017-9588-x C5714851!5714851!29022161     free     free     free Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Purinergic+Signal 2017 ; 13 (4): 611-27 Nephropedia Template TP {{tp|p=29022161|t=2017. POM-1 inhibits P2 receptors and exhibits anti-inflammatory effects in macrophages |pdf=|usr=}}{{29022161}} gab.com Text POM-1 inhibits P2 receptors and exhibits anti-inflammatory effects in macrophages JO: Purinergic Signal http://www.kidney.de/pget.php?&tw=1&pmid=29022161 #FOAvip Extracellular nucleotides can modulate the immunological response by activating purinergic receptors (P2Rs) on the cell surface of macrophages, dendritic, and other immune cells. In particular, the activation of P2X7R can induce release of cytokines and cell death as well as the uptake of large molecules through the cell membrane by a mechanism still poorly understood. Polyoxotungstate-1 (POM-1) has been proposed as a potent inhibitor of ecto-nucleotidases, enzymes that hydrolyze extracellular nucleotides, regulating the activity of P2Rs. However, the potential impact of POM-1 on P2Rs has not been evaluated. Here, we used fluorescent dye uptake, cytoplasmic free Ca2+ concentration measurement, patch-clamp recordings, scanning electron microscopy, and quantification of inflammatory mediators to investigate the effects of POM-1 on P2Rs of murine macrophages. We observed that POM-1 blocks the P2YR-dependent cytoplasmic Ca2+ increase and has partial effects on the cytoplasmic Ca2+, increasing dependence on P2XRs. POM-1 can inhibit the events related with ATP-dependent inflammasome activation, anionic dye uptake, and also the opening of large conductance channels, which are associated with P2X7R-dependent pannexin-1 activation. On the other hand, this compound has no effects on cationic fluorescent dye uptake, apoptosis, and bleb formation, also dependent on P2X7R. Moreover, POM-1 can be considered an anti-inflammatory compound, because it prevents TNF-? and nitric oxide release from LPS-treated macrophages. Twit Text FOAVip POM-1 inhibits P2 receptors and exhibits anti-inflammatory effects in macrophages ????Purinergic Signal http://www.kidney.de/pget.php?&tw=1&pmid=29022161 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=29022161 #FOAvip English Wikipedia <ref>{{Cite pmid|29022161}}</ref> POM-1 inhibits P2 receptors and exhibits anti-inflammatory effects in macrophages #MMPMID29022161 Pimenta-dos-Reis G; Torres EJL; Quintana PG; Vidal LO; dos Santos BAF; Lin CS; Heise N; Persechini PM; Schachter J Purinergic Signal 2017[Dec]; 13 (4): 611-27 PMID29022161show ga Extracellular nucleotides can modulate the immunological response by activating purinergic receptors (P2Rs) on the cell surface of macrophages, dendritic, and other immune cells. In particular, the activation of P2X7R can induce release of cytokines and cell death as well as the uptake of large molecules through the cell membrane by a mechanism still poorly understood. Polyoxotungstate-1 (POM-1) has been proposed as a potent inhibitor of ecto-nucleotidases, enzymes that hydrolyze extracellular nucleotides, regulating the activity of P2Rs. However, the potential impact of POM-1 on P2Rs has not been evaluated. Here, we used fluorescent dye uptake, cytoplasmic free Ca2+ concentration measurement, patch-clamp recordings, scanning electron microscopy, and quantification of inflammatory mediators to investigate the effects of POM-1 on P2Rs of murine macrophages. We observed that POM-1 blocks the P2YR-dependent cytoplasmic Ca2+ increase and has partial effects on the cytoplasmic Ca2+, increasing dependence on P2XRs. POM-1 can inhibit the events related with ATP-dependent inflammasome activation, anionic dye uptake, and also the opening of large conductance channels, which are associated with P2X7R-dependent pannexin-1 activation. On the other hand, this compound has no effects on cationic fluorescent dye uptake, apoptosis, and bleb formation, also dependent on P2X7R. Moreover, POM-1 can be considered an anti-inflammatory compound, because it prevents TNF-? and nitric oxide release from LPS-treated macrophages. äPOM-1 inhibits P2 receptors and exhibits anti-inflammatory effects in (epmc).pdf DeepDyve Pubget Overpricing