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10.2144/000114547

http://scihub22266oqcxt.onion/10.2144/000114547
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C5709033!5709033!28528576
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suck abstract from ncbi


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pmid28528576      Biotechniques 2017 ; 62 (5): 229-31
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  • Using non-thermal irreversible electroporation to create an in vivo niche for exogenous cell engraftment #MMPMID28528576
  • Chang TT; Zhou VX; Rubinsky B
  • Biotechniques 2017[May]; 62 (5): 229-31 PMID28528576show ga
  • The critical shortage of donor organs has spurred the investigation of alternative approaches to generate replacement organs or implant exogenous cells for treatment of end-stage organ failure. Non-thermal irreversible electroporation (NTIRE), which uses brief high electrical fields to induce irreversible permeabilization of cell membranes, has emerged as a technique for tumor ablation. This study introduces a new application for NTIRE that employs in situ cell ablation with NTIRE to create a niche within a solid organ for engraftment of exogenous cells in vivo. We treated the liver of mice with NTIRE and subsequently implanted exogenous congenic hepatocytes within the zone of cell ablation. Donor hepatocytes engrafted and integrated with host liver parenchyma that was pre-treated with NTIRE. This new approach may have value in studying the effects of a native matrix scaffold on in vivo cell growth and may pioneer a new aspect of minimally-invasive regenerative surgery.
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