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10.1002/advs.201700175 http://scihub22266oqcxt.onion/10.1002/advs.201700175 C5700650!5700650!29201613     free     free     free Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 253.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 253.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Adv+Sci+(Weinh) 2017 ; 4 (11): ä Nephropedia Template TP {{tp|p=29201613|t=2017. Genome Editing for Cancer Therapy: Delivery of Cas9 Protein/sgRNA Plasmid via a Gold Nanocluster/Lipid Core?Shell Nanocarrier |pdf=|usr=}}{{29201613}} gab.com Text Genome Editing for Cancer Therapy: Delivery of Cas9 Protein/sgRNA Plasmid via a Gold Nanocluster/Lipid Core Shell Nanocarrier JO: Adv Sci (Weinh) http://www.kidney.de/pget.php?&tw=1&pmid=29201613 #FOAvip The type II bacterial clustered, regularly interspaced, short palindromic repeats (CRISPR)?Cas9 (CRISPR?associated protein) system (CRISPR?Cas9) is a powerful toolbox for gene?editing, however, the nonviral delivery of CRISPR?Cas9 to cells or tissues remains a key challenge. This paper reports a strategy to deliver Cas9 protein and single guide RNA (sgRNA) plasmid by a nanocarrier with a core of gold nanoclusters (GNs) and a shell of lipids. By modifying the GNs with HIV?1?transactivator of transcription peptide, the cargo (Cas9/sgRNA) can be delivered into cell nuclei. This strategy is utilized to treat melanoma by designing sgRNA targeting Polo?like kinase?1 (Plk1) of the tumor. The nanoparticle (polyethylene glycol?lipid/GNs/Cas9 protein/sgPlk1 plasmid, LGCP) leads to >70% down?regulation of Plk1 protein expression of A375 cells in vitro. Moreover, the LGCP suppresses melanoma progress by 75% on mice. Thus, this strategy can deliver protein?nucleic acid hybrid agents for gene therapy. Twit Text FOAVip Genome Editing for Cancer Therapy: Delivery of Cas9 Protein/sgRNA Plasmid via a Gold Nanocluster/Lipid Core Shell Nanocarrier ????Adv Sci (Weinh) http://www.kidney.de/pget.php?&tw=1&pmid=29201613 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=29201613 #FOAvip English Wikipedia <ref>{{Cite pmid|29201613}}</ref> Genome Editing for Cancer Therapy: Delivery of Cas9 Protein/sgRNA Plasmid via a Gold Nanocluster/Lipid Core?Shell Nanocarrier #MMPMID29201613 Wang P; Zhang L; Xie Y; Wang N; Tang R; Zheng W; Jiang X Adv Sci (Weinh) 2017[Nov]; 4 (11): ä PMID29201613show ga The type II bacterial clustered, regularly interspaced, short palindromic repeats (CRISPR)?Cas9 (CRISPR?associated protein) system (CRISPR?Cas9) is a powerful toolbox for gene?editing, however, the nonviral delivery of CRISPR?Cas9 to cells or tissues remains a key challenge. This paper reports a strategy to deliver Cas9 protein and single guide RNA (sgRNA) plasmid by a nanocarrier with a core of gold nanoclusters (GNs) and a shell of lipids. By modifying the GNs with HIV?1?transactivator of transcription peptide, the cargo (Cas9/sgRNA) can be delivered into cell nuclei. This strategy is utilized to treat melanoma by designing sgRNA targeting Polo?like kinase?1 (Plk1) of the tumor. The nanoparticle (polyethylene glycol?lipid/GNs/Cas9 protein/sgPlk1 plasmid, LGCP) leads to >70% down?regulation of Plk1 protein expression of A375 cells in vitro. Moreover, the LGCP suppresses melanoma progress by 75% on mice. Thus, this strategy can deliver protein?nucleic acid hybrid agents for gene therapy. äGenome Editing for Cancer Therapy: Delivery of Cas9 Protein/sgRNA Plas(epmc).pdf DeepDyve Pubget Overpricing