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10.21769/BioProtoc.1172 http://scihub22266oqcxt.onion/10.21769/BioProtoc.1172 C5697738!5697738!29170744     free     free     free       Bio+Protoc 2014 ; 4 (13): ä Nephropedia Template TP {{tp|p=29170744|t=2014. Murine in vivo CD8+ T Cell Killing Assay |pdf=|usr=}}{{29170744}} gab.com Text Murine in vivo CD8+ T Cell Killing Assay JO: Bio Protoc http://www.kidney.de/pget.php?&tw=1&pmid=29170744 #FOAvip Antigen-specific killing ability of effector CD8+ T cells is critical for protective immunity against infection. Here, we describe in vivo cytotoxic T cell assay to examine effector function of antigen-specific CD8+ T cells. Mice infected with Listeria monocytogenes (L. monocytogenes) expressing chicken ovalbumin as a model antigen mount ovalbumin-specific CD8+ T cell responses. Effector CD8+ T cell function in vivo is determined by mixed transfer of OVA peptide-pulsed target cells with control target cells into the previously immunized mice. Difference in CFSE expression levels clearly marks two distinct populations: Antigen-pulsed target cells-CFSElow vs. unpulsed target cells-CFSEhi. The frequencies between antigen-pulsed target cells and control target cells are used as readouts of antigen-specific killing. Twit Text FOAVip Murine in vivo CD8+ T Cell Killing Assay ????Bio Protoc http://www.kidney.de/pget.php?&tw=1&pmid=29170744 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=29170744 #FOAvip English Wikipedia <ref>{{Cite pmid|29170744}}</ref> Murine in vivo CD8+ T Cell Killing Assay #MMPMID29170744 Kim MV; Ouyang W; Liao W; Zhang MQ; Li MO Bio Protoc 2014[Jul]; 4 (13): ä PMID29170744show ga Antigen-specific killing ability of effector CD8+ T cells is critical for protective immunity against infection. Here, we describe in vivo cytotoxic T cell assay to examine effector function of antigen-specific CD8+ T cells. Mice infected with Listeria monocytogenes (L. monocytogenes) expressing chicken ovalbumin as a model antigen mount ovalbumin-specific CD8+ T cell responses. Effector CD8+ T cell function in vivo is determined by mixed transfer of OVA peptide-pulsed target cells with control target cells into the previously immunized mice. Difference in CFSE expression levels clearly marks two distinct populations: Antigen-pulsed target cells-CFSElow vs. unpulsed target cells-CFSEhi. The frequencies between antigen-pulsed target cells and control target cells are used as readouts of antigen-specific killing. äMurine in vivo CD8+ T Cell Killing Assay (epmc).pdf DeepDyve Pubget Overpricing