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2017 ; 8
(ä): 1552
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CRISPRi Induced Suppression of Fimbriae Gene (fimH) of a Uropathogenic
Escherichia coli: An Approach to Inhibit Microbial Biofilms
#MMPMID29181009
Zuberi A
; Ahmad N
; Khan AU
Front Immunol
2017[]; 8
(ä): 1552
PMID29181009
show ga
Urinary tract infection (UTI) is one the common infections caused by the
recalcitrant nature of biofilms, developed after the pathogen has adhered to the
inner lining of the urinary tract. Although significant research has been made in
recent years to control these types of infection, but as of yet, no approach has
sufficiently been able to reduce the prevalence of UTIs. The main objective of
this study was to prevent UTIs through targeting the fimH gene, which is the
major virulent factor responsible for biofilm formation. The novelty of this work
lies in the use of CRISPRi, a gene specific editing tool to control such types of
infections. Accordingly, the system was designed to target fimH gene, responsible
for bacterial adherence and this approach was successfully validated by
performing microscopic, biofilm and adherence assays.