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10.1002/sctm.17-0057 http://scihub22266oqcxt.onion/10.1002/sctm.17-0057 C5689780!5689780!28618139     free     free     free Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Stem+Cells+Transl+Med 2017 ; 6 (8): 1698-709 Nephropedia Template TP {{tp|p=28618139|t=2017. Large?Scale Ex Vivo Generation of Human Red Blood Cells from Cord Blood CD34+ Cells |pdf=|usr=}}{{28618139}} gab.com Text Large Scale Ex Vivo Generation of Human Red Blood Cells from Cord Blood CD34+ Cells JO: Stem Cells Transl Med http://www.kidney.de/pget.php?&tw=1&pmid=28618139 #FOAvip The ex vivo generation of human red blood cells on a large scale from hematopoietic stem and progenitor cells has been considered as a potential method to overcome blood supply shortages. Here, we report that functional human erythrocytes can be efficiently produced from cord blood (CB) CD34+ cells using a bottle turning device culture system. Safety and efficiency studies were performed in murine and nonhuman primate (NHP) models. With the selected optimized culture conditions, one human CB CD34+ cell could be induced ex vivo to produce up to 200 million erythrocytes with a purity of 90.1%?±?6.2% and 50%?±?5.7% (mean?±?SD) for CD235a+ cells and enucleated cells, respectively. The yield of erythrocytes from one CB unit (5 million CD34+ cells) could be, in theory, equivalent to 500 blood transfusion units in clinical application. Moreover, induced human erythrocytes had normal hemoglobin content and could continue to undergo terminal maturation in the murine xenotransplantation model. In NHP model, xenotransplantation of induced human erythrocytes enhanced hematological recovery and ameliorated the hypoxia situation in the primates with hemorrhagic anemia. These findings suggested that the ex vivo?generated erythrocytes could be an alternative blood source for traditional transfusion products in the clinic. Stem Cells Translational Medicine2017;6:1698?1709 Twit Text FOAVip Large Scale Ex Vivo Generation of Human Red Blood Cells from Cord Blood CD34+ Cells ????Stem Cells Transl Med http://www.kidney.de/pget.php?&tw=1&pmid=28618139 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28618139 #FOAvip English Wikipedia <ref>{{Cite pmid|28618139}}</ref> Large?Scale Ex Vivo Generation of Human Red Blood Cells from Cord Blood CD34+ Cells #MMPMID28618139 Zhang Y; Wang C; Wang L; Shen B; Guan X; Tian J; Ren Z; Ding X; Ma Y; Dai W; Jiang Y Stem Cells Transl Med 2017[Aug]; 6 (8): 1698-709 PMID28618139show ga The ex vivo generation of human red blood cells on a large scale from hematopoietic stem and progenitor cells has been considered as a potential method to overcome blood supply shortages. Here, we report that functional human erythrocytes can be efficiently produced from cord blood (CB) CD34+ cells using a bottle turning device culture system. Safety and efficiency studies were performed in murine and nonhuman primate (NHP) models. With the selected optimized culture conditions, one human CB CD34+ cell could be induced ex vivo to produce up to 200 million erythrocytes with a purity of 90.1%?±?6.2% and 50%?±?5.7% (mean?±?SD) for CD235a+ cells and enucleated cells, respectively. The yield of erythrocytes from one CB unit (5 million CD34+ cells) could be, in theory, equivalent to 500 blood transfusion units in clinical application. Moreover, induced human erythrocytes had normal hemoglobin content and could continue to undergo terminal maturation in the murine xenotransplantation model. In NHP model, xenotransplantation of induced human erythrocytes enhanced hematological recovery and ameliorated the hypoxia situation in the primates with hemorrhagic anemia. These findings suggested that the ex vivo?generated erythrocytes could be an alternative blood source for traditional transfusion products in the clinic. Stem Cells Translational Medicine2017;6:1698?1709 äLarge?Scale Ex Vivo Generation of Human Red Blood Cells from Cord Bloo(epmc).pdf DeepDyve Pubget Overpricing