Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=29137640
&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests
in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215
Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 269.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 269.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 269.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 269.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 269.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\29137640
.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117 J+Nanobiotechnology
2017 ; 15
(1
): 82
Nephropedia Template TP
gab.com Text
Twit Text FOAVip
Twit Text #
English Wikipedia
Direct reprogramming of mouse fibroblasts into neural cells via Porphyra
yezoensis polysaccharide based high efficient gene co-delivery
#MMPMID29137640
Yu Q
; Chen J
; Deng W
; Cao X
; Wang Y
; Zhou J
; Xu W
; Du P
; Wang Q
; Yu J
; Xu X
J Nanobiotechnology
2017[Nov]; 15
(1
): 82
PMID29137640
show ga
BACKGROUND: The cell source for transplantation therapy is always a prerequisite
question to be solved in clinical applications. Neural cells are considered
non-regenerable, which highly restrict their application in the treatment for
nerve injury. Therefore, neural trans-differentiation based on gene transfection
provides a new solution to this issue. Compared to viral strategy, non-viral gene
delivery systems are considered as a more promising way to achieve this aim. This
study centers on a novel application of Porphyra yezoensis polysaccharide as a
non-viral gene carrier for the neural trans-differentiation of mouse fibroblasts.
RESULTS: Ethanediamine modified P. yezoensis polysaccharide (Ed-PYP) served as a
gene carrier and a group of plasmids that encode Ascl1, Brn4, and Tcf3 (pABT)
self-assembled into nanoparticles. Results demonstrated that Ed-PYP-pABT
nanoparticles at Ed-PYP: pABT weight ratio of 40:1 was the optimal candidate for
gene delivery. ELISA assay revealed the highest expression levels of NGF, BDNF
and SHH at 14 days after last transfection. Immunofluorescence and western blot
assays also showed robust expression of neural markers including Nestin, GFAP,
?-3tubulin, NF200, GAP43 and MAP2, in induced 3T6 cells at this time point.
CONCLUSION: Overall, these findings indicated that the P. yezoensis
polysaccharide-based non-viral gene co-delivery system is a promising strategy
for the generation of neural cells, which might facilitate the developments in
the recovery of neural injuries.
free
free
free
