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10.1016/j.jcte.2014.05.002 http://scihub22266oqcxt.onion/10.1016/j.jcte.2014.05.002 C5685016!5685016!29159085     free     free     free Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Clin+Transl+Endocrinol 2014 ; 1 (3): 66-72 Nephropedia Template TP {{tp|p=29159085|t=2014. Autoimmune response to AGE modified human DNA: Implications in type 1 diabetes mellitus? |pdf=|usr=}}{{29159085}} gab.com Text Autoimmune response to AGE modified human DNA: Implications in type 1 diabetes mellitus JO: J Clin Transl Endocrinol http://www.kidney.de/pget.php?&tw=1&pmid=29159085 #FOAvip Aims: Non-enzymatic glycation of DNA both in vivo and in vitro results in generation of free radicals, known as glycoxidation. Glycoxidation leads to structural perturbation of DNA resulting in generation of neo-antigenic epitopes having implication in autoimmune disorders like diabetes mellitus. In this study human placental DNA was glycated with methylglyoxal (MG) and lysine (Lys) in the presence of Cu2+ and its auto-antibody binding was probed in Type 1 diabetes patients. Methods: Glycation was carried out by incubating DNA with MG, Lys and Cu2+ for 24 h at 37 °C. Carboxyethyl deoxyguanosine (CEdG) formed in glycation reaction was studied by LC-MS and the pathway for Amadori formation was studied by ESI-MS techniques. Furthermore, binding characteristics of auto-antibodies in diabetes patients were assessed by direct binding, competitive ELISA and band shift assay. Results: DNA glycation with MG, Lys and Cu2+ results in the formation of CEdG (marker of DNA glycation) which was confirmed by LC-MS. The intermediate stages of glycation were confirmed by ESI-MS technique. Serum from diabetes patients exhibited enhanced binding and specificity for glycated DNA as compared to native form. Conclusions: Glycation of DNA has resulted in structural perturbation causing generation of neo-antigenic epitopes thus recognizing auto-antibodies in diabetes. Twit Text FOAVip Autoimmune response to AGE modified human DNA: Implications in type 1 diabetes mellitus ????J Clin Transl Endocrinol http://www.kidney.de/pget.php?&tw=1&pmid=29159085 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=29159085 #FOAvip English Wikipedia <ref>{{Cite pmid|29159085}}</ref> Autoimmune response to AGE modified human DNA: Implications in type 1 diabetes mellitus? #MMPMID29159085 Ahmad S; Uddin M; Habib S; Shahab U; Alam K; Ali A J Clin Transl Endocrinol 2014[Sep]; 1 (3): 66-72 PMID29159085show ga Aims: Non-enzymatic glycation of DNA both in vivo and in vitro results in generation of free radicals, known as glycoxidation. Glycoxidation leads to structural perturbation of DNA resulting in generation of neo-antigenic epitopes having implication in autoimmune disorders like diabetes mellitus. In this study human placental DNA was glycated with methylglyoxal (MG) and lysine (Lys) in the presence of Cu2+ and its auto-antibody binding was probed in Type 1 diabetes patients. Methods: Glycation was carried out by incubating DNA with MG, Lys and Cu2+ for 24 h at 37 °C. Carboxyethyl deoxyguanosine (CEdG) formed in glycation reaction was studied by LC-MS and the pathway for Amadori formation was studied by ESI-MS techniques. Furthermore, binding characteristics of auto-antibodies in diabetes patients were assessed by direct binding, competitive ELISA and band shift assay. Results: DNA glycation with MG, Lys and Cu2+ results in the formation of CEdG (marker of DNA glycation) which was confirmed by LC-MS. The intermediate stages of glycation were confirmed by ESI-MS technique. Serum from diabetes patients exhibited enhanced binding and specificity for glycated DNA as compared to native form. Conclusions: Glycation of DNA has resulted in structural perturbation causing generation of neo-antigenic epitopes thus recognizing auto-antibodies in diabetes. äAutoimmune response to AGE modified human DNA: Implications in type 1 (epmc).pdf DeepDyve Pubget Overpricing