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10.3389/fimmu.2017.01498 http://scihub22266oqcxt.onion/10.3389/fimmu.2017.01498 C5684128!5684128!29170663     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Front+Immunol 2017 ; 8 (ä): ä Nephropedia Template TP {{tp|p=29170663|t=2017. Neutrophil Functional Heterogeneity: Identification of Competitive Phagocytosis |pdf=|usr=}}{{29170663}} gab.com Text Neutrophil Functional Heterogeneity: Identification of Competitive Phagocytosis JO: Front Immunol http://www.kidney.de/pget.php?&tw=1&pmid=29170663 #FOAvip Introduction: Phagocytosis by neutrophils is a key process in the innate immune response against invading microorganisms. Despite reported heterogeneity in other neutrophils functions, little is known regarding differences in phagocytosis by individual cells. Therefore, we tested the hypothesis that heterogeneity is present in the neutrophil compartment in its potency to phagocytize bacteria. Methods: Phagocytosis assays were performed in suspension with isolated neutrophils and Staphylococcus aureus expressing different fluorescent proteins at MOIs between 1 and 10. Repetitive addition of bacteria with different fluorescent proteins and MOIs was used to compare the phagocytic capacity of S. aureus-green fluorescent protein (GFP)-positive and negative neutrophils and exclude randomness. Results: The percentage and mean fluorescence intensity (MFI) of S. aureus-GFP-positive neutrophils increased with higher MOIs. The increase in MFI was due to phagocytosis of multiple bacteria per neutrophil as was confirmed by confocal imaging. Sequential phagocytosis of GFP- and mCherry-expressing S. aureus showed a non-random process, as S. aureus-GFP-positive neutrophils preferentially phagocytized S. aureus-mCherry. Conclusion: All neutrophils were able to phagocytize S. aureus, but some were much more potent than others. Therefore, at physiologically relevant MOIs these potent phagocytizing neutrophils will outcompete the uptake of bacteria by less competent cells in a process we propose to name ?competitive phagocytosis.? Twit Text FOAVip Neutrophil Functional Heterogeneity: Identification of Competitive Phagocytosis ????Front Immunol http://www.kidney.de/pget.php?&tw=1&pmid=29170663 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=29170663 #FOAvip English Wikipedia <ref>{{Cite pmid|29170663}}</ref> Neutrophil Functional Heterogeneity: Identification of Competitive Phagocytosis #MMPMID29170663 Hellebrekers P; Hietbrink F; Vrisekoop N; Leenen LPH; Koenderman L Front Immunol 2017[]; 8 (ä): ä PMID29170663show ga Introduction: Phagocytosis by neutrophils is a key process in the innate immune response against invading microorganisms. Despite reported heterogeneity in other neutrophils functions, little is known regarding differences in phagocytosis by individual cells. Therefore, we tested the hypothesis that heterogeneity is present in the neutrophil compartment in its potency to phagocytize bacteria. Methods: Phagocytosis assays were performed in suspension with isolated neutrophils and Staphylococcus aureus expressing different fluorescent proteins at MOIs between 1 and 10. Repetitive addition of bacteria with different fluorescent proteins and MOIs was used to compare the phagocytic capacity of S. aureus-green fluorescent protein (GFP)-positive and negative neutrophils and exclude randomness. Results: The percentage and mean fluorescence intensity (MFI) of S. aureus-GFP-positive neutrophils increased with higher MOIs. The increase in MFI was due to phagocytosis of multiple bacteria per neutrophil as was confirmed by confocal imaging. Sequential phagocytosis of GFP- and mCherry-expressing S. aureus showed a non-random process, as S. aureus-GFP-positive neutrophils preferentially phagocytized S. aureus-mCherry. Conclusion: All neutrophils were able to phagocytize S. aureus, but some were much more potent than others. Therefore, at physiologically relevant MOIs these potent phagocytizing neutrophils will outcompete the uptake of bacteria by less competent cells in a process we propose to name ?competitive phagocytosis.? äNeutrophil Functional Heterogeneity: Identification of Competitive Pha(epmc).pdf DeepDyve Pubget Overpricing