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Molecular, serological and in vitro culture-based characterization of Bourbon
virus, a newly described human pathogen of the genus Thogotovirus
#MMPMID26609638
Lambert AJ
; Velez JO
; Brault AC
; Calvert AE
; Bell-Sakyi L
; Bosco-Lauth AM
; Staples JE
; Kosoy OI
J Clin Virol
2015[Dec]; 73
(?): 127-132
PMID26609638
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BACKGROUND: In June of 2014, a previously healthy man from Kansas with a recent
history of tick exposure died from complications related to an illness marked by
fever, thrombocytopenia and leukopenia. An isolate was derived from the blood of
this patient during the course of diagnostic testing. This isolate was
subsequently identified as a novel orthomyxovirus of the genus Thogotovirus by
next generation sequencing and was named Bourbon virus after the patient's county
of residence. OBJECTIVES: To support research and diagnostic aims, we provide a
basic description of Bourbon virus at both the molecular and serological levels.
Furthermore, to preliminarily identify potential host and vector range
associations we have characterized the growth kinetics of Bourbon virus in a
variety of vertebrate and invertebrate cell lines. STUDY DESIGN: Bourbon virus
was subjected to next generation-high throughput sequencing, phylogenetic, and
basic structural protein analyses as well as 2-way plaque reduction
neutralization assays. Also, we inoculated a variety of cell types with Bourbon
virus and evaluated the growth kinetics by determining viral titers in the
supernatants taken from infected cells over time. RESULTS: Bourbon virus
possesses 24-82% identity at the amino acid sequence level and low serological
cross-reactivity with other Thogotoviruses. In vitro growth kinetics reveal
robust replication of Bourbon virus in mammalian and tick cells. CONCLUSIONS:
Molecular and serological characterizations identify Bourbon virus as a novel
member of the genus Thogotovirus. Results from cell culture analyses suggest an
association between Bourbon virus and mammalian and tick hosts.
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