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10.1074/jbc.M117.799676 http://scihub22266oqcxt.onion/10.1074/jbc.M117.799676 C5682968!5682968 !28939766     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=28939766 &cmd=llinks): Failed to open stream: HTTP request failed! 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Casein kinase 2-mediated phosphorylation of Brahma-related gene 1 controls myoblast proliferation and contributes to SWI/SNF complex composition |pdf=|usr=}}{{28939766 }} gab.com Text Casein kinase 2-mediated phosphorylation of Brahma-related gene 1 controls myoblast proliferation and contributes to SWI/SNF complex composition JO: J Biol Chem http://www.kidney.de/pget.php?&tw=1&pmid=28939766 #FOAvip Transcriptional regulation is modulated in part by chromatin-remodeling enzymes that control gene accessibility by altering chromatin compaction or nucleosome positioning. Brahma-related gene 1 (Brg1), a catalytic subunit of the mammalian SWI/SNF chromatin-remodeling enzymes, is required for both myoblast proliferation and differentiation, and the control of Brg1 phosphorylation by calcineurin, PKC?1, and p38 regulates the transition to differentiation. However, we hypothesized that Brg1 activity might be regulated by additional kinases. Here, we report that Brg1 is also a target of casein kinase 2 (CK2), a serine/threonine kinase, in proliferating myoblasts. We found that CK2 interacts with Brg1, and mutation of putative phosphorylation sites to non-phosphorylatable (Ser to Ala, SA) or phosphomimetic residues (Ser to Glu, SE) reduced Brg1 phosphorylation by CK2. Although BRG1-deleted myoblasts that ectopically express the SA-Brg1 mutant proliferated similarly to the parental cells or cells ectopically expressing wild-type (WT) Brg1, ectopic expression of the SE-Brg1 mutant reduced proliferation and increased cell death, similar to observations from cells lacking Brg1. Moreover, pharmacological inhibition of CK2 increased myoblast proliferation. Furthermore, the Pax7 promoter, which controls expression of a key transcription factor required for myoblast proliferation, was in an inaccessible chromatin state in the SE-Brg1 mutant, suggesting that hyperphosphorylated Brg1 cannot remodel chromatin. WT-, SA-, and SE-Brg1 exhibited distinct differences in interacting with and affecting expression of the SWI/SNF subunits Baf155 and Baf170 and displayed differential sub-nuclear localization. Our results indicate that CK2-mediated phosphorylation of Brg1 regulates myoblast proliferation and provides insight into one mechanism by which composition of the mammalian SWI/SNF enzyme complex is regulated. Twit Text FOAVip Casein kinase 2-mediated phosphorylation of Brahma-related gene 1 controls myoblast proliferation and contributes to SWI/SNF complex composition ????J Biol Chem http://www.kidney.de/pget.php?&tw=1&pmid=28939766 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28939766 #FOAvip English Wikipedia <ref>{{Cite pmid|28939766 }}</ref> Casein kinase 2-mediated phosphorylation of Brahma-related gene 1 controls myoblast proliferation and contributes to SWI/SNF complex composition #MMPMID28939766 Padilla-Benavides T ; Nasipak BT ; Paskavitz AL ; Haokip DT ; Schnabl JM ; Nickerson JA ; Imbalzano AN J Biol Chem 2017[Nov]; 292 (45 ): 18592-18607 PMID28939766 show ga Transcriptional regulation is modulated in part by chromatin-remodeling enzymes that control gene accessibility by altering chromatin compaction or nucleosome positioning. Brahma-related gene 1 (Brg1), a catalytic subunit of the mammalian SWI/SNF chromatin-remodeling enzymes, is required for both myoblast proliferation and differentiation, and the control of Brg1 phosphorylation by calcineurin, PKC?1, and p38 regulates the transition to differentiation. However, we hypothesized that Brg1 activity might be regulated by additional kinases. Here, we report that Brg1 is also a target of casein kinase 2 (CK2), a serine/threonine kinase, in proliferating myoblasts. We found that CK2 interacts with Brg1, and mutation of putative phosphorylation sites to non-phosphorylatable (Ser to Ala, SA) or phosphomimetic residues (Ser to Glu, SE) reduced Brg1 phosphorylation by CK2. Although BRG1-deleted myoblasts that ectopically express the SA-Brg1 mutant proliferated similarly to the parental cells or cells ectopically expressing wild-type (WT) Brg1, ectopic expression of the SE-Brg1 mutant reduced proliferation and increased cell death, similar to observations from cells lacking Brg1. Moreover, pharmacological inhibition of CK2 increased myoblast proliferation. Furthermore, the Pax7 promoter, which controls expression of a key transcription factor required for myoblast proliferation, was in an inaccessible chromatin state in the SE-Brg1 mutant, suggesting that hyperphosphorylated Brg1 cannot remodel chromatin. WT-, SA-, and SE-Brg1 exhibited distinct differences in interacting with and affecting expression of the SWI/SNF subunits Baf155 and Baf170 and displayed differential sub-nuclear localization. Our results indicate that CK2-mediated phosphorylation of Brg1 regulates myoblast proliferation and provides insight into one mechanism by which composition of the mammalian SWI/SNF enzyme complex is regulated. |*Gene Expression Regulation/drug effects [MESH] |*Protein Processing, Post-Translational/drug effects [MESH] |Amino Acid Substitution [MESH] |Animals [MESH] |Casein Kinase II/drug effects/genetics/*metabolism [MESH] |Cells, Cultured [MESH] |Chromosomal Proteins, Non-Histone/chemistry/*metabolism [MESH] |DNA Helicases/genetics/*metabolism [MESH] |Female [MESH] |Male [MESH] |Mice, Inbred C57BL [MESH] |Mice, Knockout [MESH] |Mutation [MESH] |Myoblasts, Skeletal/cytology/drug effects/*metabolism [MESH] |Nuclear Proteins/genetics/*metabolism [MESH] |PAX7 Transcription Factor/agonists/genetics/metabolism [MESH] |Phosphorylation/drug effects [MESH] |Promoter Regions, Genetic/drug effects [MESH] |Protein Kinase Inhibitors/pharmacology [MESH] |Protein Multimerization/drug effects [MESH] |Protein Transport/drug effects [MESH] |Recombinant Proteins/genetics/metabolism [MESH] |Satellite Cells, Skeletal Muscle/cytology/drug effects/metabolism [MESH]Casein kinase 2-mediated phosphorylation of Brahma-related gene 1 cont(epmc).pdf DeepDyve Pubget Overpricing