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10.1038/cddis.2017.511 http://scihub22266oqcxt.onion/10.1038/cddis.2017.511 C5682676!5682676 !29022900     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\29022900 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Cell+Death+Dis 2017 ; 8 (10 ): e3109 Nephropedia Template TP {{tp|p=29022900 |t=2017. Increased 15-lipoxygenase-1 expression in chondrocytes contributes to the pathogenesis of osteoarthritis |pdf=|usr=}}{{29022900 }} gab.com Text Increased 15-lipoxygenase-1 expression in chondrocytes contributes to the pathogenesis of osteoarthritis JO: Cell Death Dis http://www.kidney.de/pget.php?&tw=1&pmid=29022900 #FOAvip 15-Lipoxygenase-1 (15-LO-1) is involved in many pathological processes. The purpose of this study was to determine the potential role of 15-LO-1 in osteoarthritis (OA). The levels of 15-LO-1 expression were measured by western blotting and quantitative real-time PCR in articular cartilage from the OA rat models and OA patients. To further investigate the effects of 15-LO-1 on chondrocyte functions, such as extracellular matrix (ECM) secretion, the release of matrix-degrading enzymes, the production of reactive oxygen species (ROS), cell proliferation and apoptosis, we decreased or increased 15-LO-1 expression in chondrocytes by means of transfecting with siRNA targeting 15-LO-1 and plasmid encoding 15-LO-1, respectively. The results showed that 15-LO-1 expression was obviously increased in articular cartilage from OA rats and OA patients. It was also found that many factor-related OA, such as mechanical loading, ROS, SNP and inflammatory factor, significantly promoted 15-LO-1 expression and activity in chondrocytes. Silencing 15-LO-1 was able to markedly alleviate mechanical loading-induced cartilage ECM secretion, cartilage-degrading enzyme secretion and ROS production. Overexpression of 15-LO-1 could inhibit chondrocyte proliferation and induce chondrocyte apoptosis. In addition, reduction of 15-LO-1 in vivo significantly alleviated OA. Taken together, these results indicate that 15-LO-1 has an important role in the disease progression of OA. Thus 15-LO-1 may be a good target for developing drugs in the treatment of OA. Twit Text FOAVip Increased 15-lipoxygenase-1 expression in chondrocytes contributes to the pathogenesis of osteoarthritis ????Cell Death Dis http://www.kidney.de/pget.php?&tw=1&pmid=29022900 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=29022900 #FOAvip English Wikipedia <ref>{{Cite pmid|29022900 }}</ref> Increased 15-lipoxygenase-1 expression in chondrocytes contributes to the pathogenesis of osteoarthritis #MMPMID29022900 Chen K ; Yan Y ; Li C ; Yuan J ; Wang F ; Huang P ; Qian N ; Qi J ; Zhou H ; Zhou Q ; Deng L ; He C ; Guo L Cell Death Dis 2017[Oct]; 8 (10 ): e3109 PMID29022900 show ga 15-Lipoxygenase-1 (15-LO-1) is involved in many pathological processes. The purpose of this study was to determine the potential role of 15-LO-1 in osteoarthritis (OA). The levels of 15-LO-1 expression were measured by western blotting and quantitative real-time PCR in articular cartilage from the OA rat models and OA patients. To further investigate the effects of 15-LO-1 on chondrocyte functions, such as extracellular matrix (ECM) secretion, the release of matrix-degrading enzymes, the production of reactive oxygen species (ROS), cell proliferation and apoptosis, we decreased or increased 15-LO-1 expression in chondrocytes by means of transfecting with siRNA targeting 15-LO-1 and plasmid encoding 15-LO-1, respectively. The results showed that 15-LO-1 expression was obviously increased in articular cartilage from OA rats and OA patients. It was also found that many factor-related OA, such as mechanical loading, ROS, SNP and inflammatory factor, significantly promoted 15-LO-1 expression and activity in chondrocytes. Silencing 15-LO-1 was able to markedly alleviate mechanical loading-induced cartilage ECM secretion, cartilage-degrading enzyme secretion and ROS production. Overexpression of 15-LO-1 could inhibit chondrocyte proliferation and induce chondrocyte apoptosis. In addition, reduction of 15-LO-1 in vivo significantly alleviated OA. Taken together, these results indicate that 15-LO-1 has an important role in the disease progression of OA. Thus 15-LO-1 may be a good target for developing drugs in the treatment of OA. |Animals [MESH] |Apoptosis/physiology [MESH] |Arachidonate 15-Lipoxygenase/genetics/*metabolism [MESH] |Cartilage, Articular/cytology/*pathology [MESH] |Cell Proliferation/physiology [MESH] |Cells, Cultured [MESH] |Chondrocytes/*metabolism [MESH] |Disease Progression [MESH] |Extracellular Matrix/metabolism [MESH] |Humans [MESH] |Knee Joint/cytology/*pathology [MESH] |Male [MESH] |Osteoarthritis/*pathology [MESH] |RNA Interference [MESH] |RNA, Small Interfering/genetics [MESH] |Rats [MESH] |Rats, Sprague-Dawley [MESH]Increased 15-lipoxygenase-1 expression in chondrocytes contributes to (epmc).pdf DeepDyve Pubget Overpricing