Use my Search Websuite to scan PubMed, PMCentral, Journal Hosts and Journal Archives, FullText. Kick-your-searchterm to multiple Engines kick-your-query now !>

A dictionary by aggregated review articles of nephrology, medicine and the life sciences Your one-stop-run pathway from word to the immediate pdf of peer-reviewed on-topic knowledge.

10.1002/acn3.444 http://scihub22266oqcxt.onion/10.1002/acn3.444 C5682115!5682115!29159189     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Ann+Clin+Transl+Neurol 2017 ; 4 (11): 768-83 Nephropedia Template TP {{tp|p=29159189|t=2017. NMDAR encephalitis: passive transfer from man to mouse by a recombinant antibody |pdf=|usr=}}{{29159189}} gab.com Text NMDAR encephalitis: passive transfer from man to mouse by a recombinant antibody JO: Ann Clin Transl Neurol http://www.kidney.de/pget.php?&tw=1&pmid=29159189 #FOAvip Objective: Autoimmune encephalitis is most frequently associated with anti?NMDAR autoantibodies. Their pathogenic relevance has been suggested by passive transfer of patients' cerebrospinal fluid (CSF) in mice in vivo. We aimed to analyze the intrathecal plasma cell repertoire, identify autoantibody?producing clones, and characterize their antibody signatures in recombinant form. Methods: Patients with recent onset typical anti?NMDAR encephalitis were subjected to flow cytometry analysis of the peripheral and intrathecal immune response before, during, and after immunotherapy. Recombinant human monoclonal antibodies (rhuMab) were cloned and expressed from matching immunoglobulin heavy? (IgH) and light?chain (IgL) amplicons of clonally expanded intrathecal plasma cells (cePc) and tested for their pathogenic relevance. Results: Intrathecal accumulation of B and plasma cells corresponded to the clinical course. The presence of cePc with hypermutated antigen receptors indicated an antigen?driven intrathecal immune response. Consistently, a single recombinant human GluN1?specific monoclonal antibody, rebuilt from intrathecal cePc, was sufficient to reproduce NMDAR epitope specificity in vitro. After intraventricular infusion in mice, it accumulated in the hippocampus, decreased synaptic NMDAR density, and caused severe reversible memory impairment, a key pathogenic feature of the human disease, in vivo. Interpretation: A CNS?specific humoral immune response is present in anti?NMDAR encephalitis specifically targeting the GluN1 subunit of the NMDAR. Using reverse genetics, we recovered the typical intrathecal antibody signature in recombinant form, and proved its pathogenic relevance by passive transfer of disease symptoms from man to mouse, providing the critical link between intrathecal immune response and the pathogenesis of anti?NMDAR encephalitis as a humorally mediated autoimmune disease. Twit Text FOAVip NMDAR encephalitis: passive transfer from man to mouse by a recombinant antibody ????Ann Clin Transl Neurol http://www.kidney.de/pget.php?&tw=1&pmid=29159189 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=29159189 #FOAvip English Wikipedia <ref>{{Cite pmid|29159189}}</ref> NMDAR encephalitis: passive transfer from man to mouse by a recombinant antibody #MMPMID29159189 Malviya M; Barman S; Golombeck KS; Planagumŕ J; Mannara F; Strutz?Seebohm N; Wrzos C; Demir F; Baksmeier C; Steckel J; Falk KK; Gross CC; Kovac S; Bönte K; Johnen A; Wandinger K; Martín?García E; Becker AJ; Elger CE; Klöcker N; Wiendl H; Meuth SG; Hartung H; Seebohm G; Leypoldt F; Maldonado R; Stadelmann C; Dalmau J; Melzer N; Goebels N Ann Clin Transl Neurol 2017[Nov]; 4 (11): 768-83 PMID29159189show ga Objective: Autoimmune encephalitis is most frequently associated with anti?NMDAR autoantibodies. Their pathogenic relevance has been suggested by passive transfer of patients' cerebrospinal fluid (CSF) in mice in vivo. We aimed to analyze the intrathecal plasma cell repertoire, identify autoantibody?producing clones, and characterize their antibody signatures in recombinant form. Methods: Patients with recent onset typical anti?NMDAR encephalitis were subjected to flow cytometry analysis of the peripheral and intrathecal immune response before, during, and after immunotherapy. Recombinant human monoclonal antibodies (rhuMab) were cloned and expressed from matching immunoglobulin heavy? (IgH) and light?chain (IgL) amplicons of clonally expanded intrathecal plasma cells (cePc) and tested for their pathogenic relevance. Results: Intrathecal accumulation of B and plasma cells corresponded to the clinical course. The presence of cePc with hypermutated antigen receptors indicated an antigen?driven intrathecal immune response. Consistently, a single recombinant human GluN1?specific monoclonal antibody, rebuilt from intrathecal cePc, was sufficient to reproduce NMDAR epitope specificity in vitro. After intraventricular infusion in mice, it accumulated in the hippocampus, decreased synaptic NMDAR density, and caused severe reversible memory impairment, a key pathogenic feature of the human disease, in vivo. Interpretation: A CNS?specific humoral immune response is present in anti?NMDAR encephalitis specifically targeting the GluN1 subunit of the NMDAR. Using reverse genetics, we recovered the typical intrathecal antibody signature in recombinant form, and proved its pathogenic relevance by passive transfer of disease symptoms from man to mouse, providing the critical link between intrathecal immune response and the pathogenesis of anti?NMDAR encephalitis as a humorally mediated autoimmune disease. äNMDAR encephalitis: passive transfer from man to mouse by a recombinan(epmc).pdf DeepDyve Pubget Overpricing