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10.1016/j.molmet.2017.08.005 http://scihub22266oqcxt.onion/10.1016/j.molmet.2017.08.005 C5681241!5681241 !29107288     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\29107288 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Mol+Metab 2017 ; 6 (11 ): 1407-1418 Nephropedia Template TP {{tp|p=29107288 |t=2017. Identification of islet-enriched long non-coding RNAs contributing to ?-cell failure in type 2 diabetes |pdf=|usr=}}{{29107288 }} gab.com Text Identification of islet-enriched long non-coding RNAs contributing to -cell failure in type 2 diabetes JO: Mol Metab http://www.kidney.de/pget.php?&tw=1&pmid=29107288 #FOAvip OBJECTIVE: Non-coding RNAs constitute a major fraction of the ?-cell transcriptome. While the involvement of microRNAs is well established, the contribution of long non-coding RNAs (lncRNAs) in the regulation of ?-cell functions and in diabetes development remains poorly understood. The aim of this study was to identify novel islet lncRNAs differently expressed in type 2 diabetes models and to investigate their role in ?-cell failure and in the development of the disease. METHODS: Novel transcripts dysregulated in the islets of diet-induced obese mice were identified by high throughput RNA-sequencing coupled with de novo annotation. Changes in the level of the lncRNAs were assessed by real-time PCR. The functional role of the selected lncRNAs was determined by modifying their expression in MIN6 cells and primary islet cells. RESULTS: We identified about 1500 novel lncRNAs, a number of which were differentially expressed in obese mice. The expression of two lncRNAs highly enriched in ?-cells, ?linc2, and ?linc3, correlated to body weight gain and glycemia levels in obese mice and was also modified in diabetic db/db mice. The expression of both lncRNAs was also modulated in vitro in isolated islet cells by glucolipotoxic conditions. Moreover, the expression of the human orthologue of ?linc3 was altered in the islets of type 2 diabetic patients and was associated to the BMI of the donors. Modulation of the level of ?linc2 and ?linc3 by overexpression or downregulation in MIN6 and mouse islet cells did not affect insulin secretion but increased ?-cell apoptosis. CONCLUSIONS: Taken together, the data show that lncRNAs are modulated in a model of obesity-associated type 2 diabetes and that variations in the expression of some of them may contribute to ?-cell failure during the development of the disease. Twit Text FOAVip Identification of islet-enriched long non-coding RNAs contributing to -cell failure in type 2 diabetes ????Mol Metab http://www.kidney.de/pget.php?&tw=1&pmid=29107288 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=29107288 #FOAvip English Wikipedia <ref>{{Cite pmid|29107288 }}</ref> Identification of islet-enriched long non-coding RNAs contributing to ?-cell failure in type 2 diabetes #MMPMID29107288 Motterle A ; Gattesco S ; Peyot ML ; Esguerra JLS ; Gomez-Ruiz A ; Laybutt DR ; Gilon P ; Burdet F ; Ibberson M ; Eliasson L ; Prentki M ; Regazzi R Mol Metab 2017[Nov]; 6 (11 ): 1407-1418 PMID29107288 show ga OBJECTIVE: Non-coding RNAs constitute a major fraction of the ?-cell transcriptome. While the involvement of microRNAs is well established, the contribution of long non-coding RNAs (lncRNAs) in the regulation of ?-cell functions and in diabetes development remains poorly understood. The aim of this study was to identify novel islet lncRNAs differently expressed in type 2 diabetes models and to investigate their role in ?-cell failure and in the development of the disease. METHODS: Novel transcripts dysregulated in the islets of diet-induced obese mice were identified by high throughput RNA-sequencing coupled with de novo annotation. Changes in the level of the lncRNAs were assessed by real-time PCR. The functional role of the selected lncRNAs was determined by modifying their expression in MIN6 cells and primary islet cells. RESULTS: We identified about 1500 novel lncRNAs, a number of which were differentially expressed in obese mice. The expression of two lncRNAs highly enriched in ?-cells, ?linc2, and ?linc3, correlated to body weight gain and glycemia levels in obese mice and was also modified in diabetic db/db mice. The expression of both lncRNAs was also modulated in vitro in isolated islet cells by glucolipotoxic conditions. Moreover, the expression of the human orthologue of ?linc3 was altered in the islets of type 2 diabetic patients and was associated to the BMI of the donors. Modulation of the level of ?linc2 and ?linc3 by overexpression or downregulation in MIN6 and mouse islet cells did not affect insulin secretion but increased ?-cell apoptosis. CONCLUSIONS: Taken together, the data show that lncRNAs are modulated in a model of obesity-associated type 2 diabetes and that variations in the expression of some of them may contribute to ?-cell failure during the development of the disease. |Animals [MESH] |Diabetes Mellitus, Type 2/*genetics/metabolism [MESH] |Diet, High-Fat [MESH] |Disease Models, Animal [MESH] |Gene Expression/genetics [MESH] |Insulin-Secreting Cells/metabolism [MESH] |Insulin/metabolism [MESH] |Islets of Langerhans/metabolism [MESH] |Male [MESH] |Mice [MESH] |Mice, Inbred C57BL [MESH] |Mice, Obese [MESH] |RNA, Long Noncoding/genetics/*metabolism [MESH] |Sequence Analysis, RNA [MESH]Identification of islet-enriched long non-coding RNAs contributing to (epmc).pdf DeepDyve Pubget Overpricing