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10.1080/20002297.2017.1385369

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suck abstract from ncbi


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pmid29152157      J+Oral+Microbiol 2017 ; 9 (1): ä
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  • A dysbiotic mycobiome dominated by Candida albicans is identified within oral squamous-cell carcinomas #MMPMID29152157
  • Perera M; Al-hebshi NN; Perera I; Ipe D; Ulett GC; Speicher DJ; Chen T; Johnson NW
  • J Oral Microbiol 2017[]; 9 (1): ä PMID29152157show ga
  • The aim of this study was to characterize the mycobiome associated with oral squamous-cell carcinoma (OSCC). DNA was extracted from 52 tissue biopsies (cases: 25 OSCC; controls: 27 intra-oral fibro-epithelial polyps [FEP]) and sequenced for the fungal internal transcribed spacer 2 region using Illumina? 2 x300bp chemistry. Merged reads were classified to species level using a BLASTN-algorithm with UNITE?s named species sequences as reference. Downstream analyses were performed using QIIME? and linear discriminant analysis effect size. A total of 364 species representing 160 genera and two phyla (Ascomycota and Basidiomycota) were identified, with Candida and Malassezia making up 48% and 11% of the average mycobiome, respectively. However, only five species and four genera were detected in ?50% of the samples. The species richness and diversity were significantly lower in OSCC. Genera Candida, Hannaella, and Gibberella were overrepresented in OSCC; Alternaria and Trametes were more abundant in FEP. Species-wise, Candida albicans, Candida etchellsii, and a Hannaella luteola?like species were enriched in OSCC, while aHanseniaspora uvarum?like species, Malassezia restricta, and Aspergillus tamarii were the most significantly abundant in FEP. In conclusion, a dysbiotic mycobiome dominated by C. albicans was found in association with OSCC, a finding worth further investigation.
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