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2017 ; 23
(40
): 7242-7252
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Dachaihu decoction ameliorates pancreatic fibrosis by inhibiting macrophage
infiltration in chronic pancreatitis
#MMPMID29142471
Duan LF
; Xu XF
; Zhu LJ
; Liu F
; Zhang XQ
; Wu N
; Fan JW
; Xin JQ
; Zhang H
World J Gastroenterol
2017[Oct]; 23
(40
): 7242-7252
PMID29142471
show ga
AIM: To explore the role of macrophages in chronic pancreatitis (CP) and the
effect of Dachaihu decoction (DCHD) on pancreatic fibrosis in mice. METHODS:
KunMing mice were randomly divided into a control group, CP group, and DCHD
group. In the CP and DCHD groups, mice were intraperitoneally injected with 20%
L-arginine (3 g/kg twice 1 d/wk for 6 wk). Mice in the DCHD group were
administered DCHD intragastrically at a dose of 14 g/kg/d 1 wk after CP
induction. At 2 wk, 4 wk and 6 wk post-modeling, the morphology of the pancreas
was observed using hematoxylin and eosin, and Masson staining. Interleukin-6
(IL-6) serum levels were assayed using an enzyme-linked immunosorbent assay.
Double immunofluorescence staining was performed to observe the co-expression of
F4/80 and IL-6 in the pancreas. Inflammatory factors including monocyte
chemoattractant protein-1 (MCP-1), macrophage inflammatory protein-1? (MIP-1?)
and IL-6 were determined using real time-polymerase chain reaction. Western blot
analysis was used to detect fibronectin levels in the pancreas. RESULTS: Compared
with the control group, mice with 20% L-arginine-induced CP had obvious
macrophage infiltration and a higher level of fibrosis. IL-6 serum concentrations
were significantly increased. Double immunofluorescence staining showed that IL-6
and F4/80 were co-expressed in the pancreas. With the administration of DCHD, the
infiltration of macrophages and degree of fibrosis in the pancreas were
significantly attenuated; IL-6, MCP-1 and MIP-1? mRNA, and fibronectin levels
were reduced. CONCLUSION: The dominant role of macrophages in the development of
CP was mainly related to IL-6 production. DCHD was effective in ameliorating
pancreatic fibrosis by inhibiting macrophage infiltration and inflammatory factor
secretion in the pancreas.
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