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Deprecated: Implicit conversion from float 243.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Medicine+(Baltimore) 2017 ; 96 (43): ä Nephropedia Template TP
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Effects of TRPC1 on epithelial mesenchymal transition in human airway in chronic obstructive pulmonary disease #MMPMID29068985
Xu F; Liu XC; Li L; Ma CN; Zhang YJ
Medicine (Baltimore) 2017[Oct]; 96 (43): ä PMID29068985show ga
Background:: We investigated the effects of TRPC1 on epithelial mesenchymal transition (EMT) in human airway in chronic obstructive pulmonary disease (COPD). Methods:: A total of 94 patients who underwent lobectomy were selected and divided into COPD (49 cases) and control (45 cases) groups. Immunohistochemistry was applied to detect expression of E-cadherin and vimentin and TRPC1. Correlation of TRPC1 expression with E-cadherin and vimentin expression, and correlations of lung function indicators in COPD patients with expression of TRPC1, E-cadherin, and vimentin were analyzed. Human airway epithelial cells (16HBE) were used for cell experiments; and cigarette smoking extract (CSE) was adopted to establish the COPD model using TRPC1 recombinant plasmids and siRNA. Cells were assigned into the control, CSE, CSE?+?vector, CSE?+?TRPC1, CSE?+?si-NC, and CSE?+?si-TRPC1 groups. Quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot were implemented to detect expression of TRPC1, E-cadherin, and vimentin. Results:: Compared with the control group, expression of TRPC1 and vimentin significantly increased while expression of E-cadherin decreased in the COPD group, and protein expression of TRPC1 was positively correlated with the protein expression of vimentin but negatively correlated with the protein expression of E-cadherin. Patients exhibiting positive expression of TRPC1 had lower FEV1, FEV1%Pred, and FEV1/FVC, compared with the patients exhibiting negative expression of TRPC1. Compared with the control group, expression of TRPC1 and vimentin increased, whereas expression of E-cadherin decreased in the CSE, CSE?+?vector, CSE?+?TRPC1, and CSE?+?si-NC groups. Compared with the CSE and CSE?+?vector groups, the expression of TRPC1 and vimentin increased but the expression of E-cadherin decreased in the CSE?+?TRPC1 group. Compared with the CSE and CSE?+?si-NC groups, the expression of TRPC1 and vimentin decreased but the expression of E-cadherin increased in the CSE?+?si-TRPC1 group. No significant differences were observed among the CSE, CSE?+?vector and CSE?+?si-NC groups. Conclusion:: Overexpression of TRPC1 in COPD promoted EMT process and TRPC1 may be a new and interesting focus for COPD new treatment in the future.