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10.1097/MD.0000000000008166 http://scihub22266oqcxt.onion/10.1097/MD.0000000000008166 C5671818!5671818 !29068985     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 243.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 243.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\29068985 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Medicine+(Baltimore) 2017 ; 96 (43 ): e8166 Nephropedia Template TP {{tp|p=29068985 |t=2017. Effects of TRPC1 on epithelial mesenchymal transition in human airway in chronic obstructive pulmonary disease |pdf=|usr=}}{{29068985 }} gab.com Text Effects of TRPC1 on epithelial mesenchymal transition in human airway in chronic obstructive pulmonary disease JO: Medicine (Baltimore) http://www.kidney.de/pget.php?&tw=1&pmid=29068985 #FOAvip BACKGROUND: We investigated the effects of TRPC1 on epithelial mesenchymal transition (EMT) in human airway in chronic obstructive pulmonary disease (COPD). METHODS: A total of 94 patients who underwent lobectomy were selected and divided into COPD (49 cases) and control (45 cases) groups. Immunohistochemistry was applied to detect expression of E-cadherin and vimentin and TRPC1. Correlation of TRPC1 expression with E-cadherin and vimentin expression, and correlations of lung function indicators in COPD patients with expression of TRPC1, E-cadherin, and vimentin were analyzed. Human airway epithelial cells (16HBE) were used for cell experiments; and cigarette smoking extract (CSE) was adopted to establish the COPD model using TRPC1 recombinant plasmids and siRNA. Cells were assigned into the control, CSE, CSE?+?vector, CSE?+?TRPC1, CSE?+?si-NC, and CSE?+?si-TRPC1 groups. Quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot were implemented to detect expression of TRPC1, E-cadherin, and vimentin. RESULTS: Compared with the control group, expression of TRPC1 and vimentin significantly increased while expression of E-cadherin decreased in the COPD group, and protein expression of TRPC1 was positively correlated with the protein expression of vimentin but negatively correlated with the protein expression of E-cadherin. Patients exhibiting positive expression of TRPC1 had lower FEV1, FEV1%Pred, and FEV1/FVC, compared with the patients exhibiting negative expression of TRPC1. Compared with the control group, expression of TRPC1 and vimentin increased, whereas expression of E-cadherin decreased in the CSE, CSE?+?vector, CSE?+?TRPC1, and CSE?+?si-NC groups. Compared with the CSE and CSE?+?vector groups, the expression of TRPC1 and vimentin increased but the expression of E-cadherin decreased in the CSE?+?TRPC1 group. Compared with the CSE and CSE?+?si-NC groups, the expression of TRPC1 and vimentin decreased but the expression of E-cadherin increased in the CSE?+?si-TRPC1 group. No significant differences were observed among the CSE, CSE?+?vector and CSE?+?si-NC groups. CONCLUSION: Overexpression of TRPC1 in COPD promoted EMT process and TRPC1 may be a new and interesting focus for COPD new treatment in the future. Twit Text FOAVip Effects of TRPC1 on epithelial mesenchymal transition in human airway in chronic obstructive pulmonary disease ????Medicine (Baltimore) http://www.kidney.de/pget.php?&tw=1&pmid=29068985 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=29068985 #FOAvip English Wikipedia <ref>{{Cite pmid|29068985 }}</ref> Effects of TRPC1 on epithelial mesenchymal transition in human airway in chronic obstructive pulmonary disease #MMPMID29068985 Xu F ; Liu XC ; Li L ; Ma CN ; Zhang YJ Medicine (Baltimore) 2017[Oct]; 96 (43 ): e8166 PMID29068985 show ga BACKGROUND: We investigated the effects of TRPC1 on epithelial mesenchymal transition (EMT) in human airway in chronic obstructive pulmonary disease (COPD). METHODS: A total of 94 patients who underwent lobectomy were selected and divided into COPD (49 cases) and control (45 cases) groups. Immunohistochemistry was applied to detect expression of E-cadherin and vimentin and TRPC1. Correlation of TRPC1 expression with E-cadherin and vimentin expression, and correlations of lung function indicators in COPD patients with expression of TRPC1, E-cadherin, and vimentin were analyzed. Human airway epithelial cells (16HBE) were used for cell experiments; and cigarette smoking extract (CSE) was adopted to establish the COPD model using TRPC1 recombinant plasmids and siRNA. Cells were assigned into the control, CSE, CSE?+?vector, CSE?+?TRPC1, CSE?+?si-NC, and CSE?+?si-TRPC1 groups. Quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot were implemented to detect expression of TRPC1, E-cadherin, and vimentin. RESULTS: Compared with the control group, expression of TRPC1 and vimentin significantly increased while expression of E-cadherin decreased in the COPD group, and protein expression of TRPC1 was positively correlated with the protein expression of vimentin but negatively correlated with the protein expression of E-cadherin. Patients exhibiting positive expression of TRPC1 had lower FEV1, FEV1%Pred, and FEV1/FVC, compared with the patients exhibiting negative expression of TRPC1. Compared with the control group, expression of TRPC1 and vimentin increased, whereas expression of E-cadherin decreased in the CSE, CSE?+?vector, CSE?+?TRPC1, and CSE?+?si-NC groups. Compared with the CSE and CSE?+?vector groups, the expression of TRPC1 and vimentin increased but the expression of E-cadherin decreased in the CSE?+?TRPC1 group. Compared with the CSE and CSE?+?si-NC groups, the expression of TRPC1 and vimentin decreased but the expression of E-cadherin increased in the CSE?+?si-TRPC1 group. No significant differences were observed among the CSE, CSE?+?vector and CSE?+?si-NC groups. CONCLUSION: Overexpression of TRPC1 in COPD promoted EMT process and TRPC1 may be a new and interesting focus for COPD new treatment in the future. |*Epithelial-Mesenchymal Transition [MESH] |Adult [MESH] |Aged [MESH] |Blotting, Western [MESH] |Cadherins/metabolism [MESH] |Cells, Cultured [MESH] |Epithelial Cells/metabolism [MESH] |Female [MESH] |Humans [MESH] |Immunohistochemistry [MESH] |Lung/metabolism/pathology/physiopathology [MESH] |Male [MESH] |Middle Aged [MESH] |Pulmonary Disease, Chronic Obstructive/*metabolism/*pathology/physiopathology [MESH] |Real-Time Polymerase Chain Reaction [MESH] |TRPC Cation Channels/*metabolism [MESH]Effects of TRPC1 on epithelial mesenchymal transition in human airway (epmc).pdf DeepDyve Pubget Overpricing