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10.1038/s41598-017-14809-8 http://scihub22266oqcxt.onion/10.1038/s41598-017-14809-8 C5665994!5665994!29093551     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Sci+Rep 2017 ; 7 (ä): ä Nephropedia Template TP {{tp|p=29093551|t=2017. Asymmetric BMP4 signalling improves the realism of kidney organoids |pdf=|usr=}}{{29093551}} gab.com Text Asymmetric BMP4 signalling improves the realism of kidney organoids JO: Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=29093551 #FOAvip We present a strategy for increasing the anatomical realism of organoids by applying asymmetric cues to mimic spatial information that is present in natural embryonic development, and demonstrate it using mouse kidney organoids. Existing methods for making kidney organoids in mice yield developing nephrons arranged around a symmetrical collecting duct tree that has no ureter. We use transplant experiments to demonstrate plasticity in the fate choice between collecting duct and ureter, and show that an environment rich in BMP4 promotes differentiation of early collecting ducts into uroplakin-positive, unbranched, ureter-like epithelial tubules. Further, we show that application of BMP4-releasing beads in one place in an organoid can break the symmetry of the system, causing a nearby collecting duct to develop into a uroplakin-positive, broad, unbranched, ureter-like ?trunk? from one end of which true collecting duct branches radiate and induce nephron development in an arrangement similar to natural kidneys. The idea of using local symmetry-breaking cues to improve the realism of organoids may have applications to organoid systems other than the kidney. Twit Text FOAVip Asymmetric BMP4 signalling improves the realism of kidney organoids ????Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=29093551 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=29093551 #FOAvip English Wikipedia <ref>{{Cite pmid|29093551}}</ref> Asymmetric BMP4 signalling improves the realism of kidney organoids #MMPMID29093551 Mills CG; Lawrence ML; Munro DAD; Elhendawi M; Mullins JJ; Davies JA Sci Rep 2017[]; 7 (ä): ä PMID29093551show ga We present a strategy for increasing the anatomical realism of organoids by applying asymmetric cues to mimic spatial information that is present in natural embryonic development, and demonstrate it using mouse kidney organoids. Existing methods for making kidney organoids in mice yield developing nephrons arranged around a symmetrical collecting duct tree that has no ureter. We use transplant experiments to demonstrate plasticity in the fate choice between collecting duct and ureter, and show that an environment rich in BMP4 promotes differentiation of early collecting ducts into uroplakin-positive, unbranched, ureter-like epithelial tubules. Further, we show that application of BMP4-releasing beads in one place in an organoid can break the symmetry of the system, causing a nearby collecting duct to develop into a uroplakin-positive, broad, unbranched, ureter-like ?trunk? from one end of which true collecting duct branches radiate and induce nephron development in an arrangement similar to natural kidneys. The idea of using local symmetry-breaking cues to improve the realism of organoids may have applications to organoid systems other than the kidney. äAsymmetric BMP4 signalling improves the realism of kidney organoids (epmc).pdf DeepDyve Pubget Overpricing