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10.1038/s41467-017-01222-y http://scihub22266oqcxt.onion/10.1038/s41467-017-01222-y C5662744!5662744!29084946     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nat+Commun 2017 ; 8 (ä): ä Nephropedia Template TP {{tp|p=29084946|t=2017. Drug-tunable multidimensional synthetic gene control using inducible degron-tagged dCas9 effectors |pdf=|usr=}}{{29084946}} gab.com Text Drug-tunable multidimensional synthetic gene control using inducible degron-tagged dCas9 effectors JO: Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=29084946 #FOAvip The nuclease-deactivated variant of CRISPR-Cas9 proteins (dCas9) fused to heterologous transactivation domains can act as a potent guide RNA sequence-directed inducer or repressor of gene expression in mammalian cells. In such a system the long-term presence of a stable dCas9 effector can be a draw-back precluding the ability to switch rapidly between repressed and activated target gene expression states, imposing a static environment on the synthetic regulatory circuits in the cell. To address this issue we have generated a toolkit of conditionally degradable or stabilisable orthologous dCas9 or Cpf1 effector proteins, thus opening options for multidimensional control of functional activities through combinations of orthogonal, drug-tunable artificial transcription factors. Twit Text FOAVip Drug-tunable multidimensional synthetic gene control using inducible degron-tagged dCas9 effectors ????Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=29084946 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=29084946 #FOAvip English Wikipedia <ref>{{Cite pmid|29084946}}</ref> Drug-tunable multidimensional synthetic gene control using inducible degron-tagged dCas9 effectors #MMPMID29084946 Kleinjan DA; Wardrope C; Nga Sou S; Rosser SJ Nat Commun 2017[]; 8 (ä): ä PMID29084946show ga The nuclease-deactivated variant of CRISPR-Cas9 proteins (dCas9) fused to heterologous transactivation domains can act as a potent guide RNA sequence-directed inducer or repressor of gene expression in mammalian cells. In such a system the long-term presence of a stable dCas9 effector can be a draw-back precluding the ability to switch rapidly between repressed and activated target gene expression states, imposing a static environment on the synthetic regulatory circuits in the cell. To address this issue we have generated a toolkit of conditionally degradable or stabilisable orthologous dCas9 or Cpf1 effector proteins, thus opening options for multidimensional control of functional activities through combinations of orthogonal, drug-tunable artificial transcription factors. äDrug-tunable multidimensional synthetic gene control using inducible d(epmc).pdf DeepDyve Pubget Overpricing