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10.1111/jcmm.13191 http://scihub22266oqcxt.onion/10.1111/jcmm.13191 C5661261!5661261 !28524456     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=28524456 &cmd=llinks): Failed to open stream: HTTP request failed! 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Endothelial microparticles released by activated protein C protect beta cells through EPCR/PAR1 and annexin A1/FPR2 pathways in islets |pdf=|usr=}}{{28524456 }} gab.com Text Endothelial microparticles released by activated protein C protect beta cells through EPCR/PAR1 and annexin A1/FPR2 pathways in islets JO: J Cell Mol Med http://www.kidney.de/pget.php?&tw=1&pmid=28524456 #FOAvip Islet transplantation is associated with early ischaemia/reperfusion, localized coagulation and redox-sensitive endothelial dysfunction. In animal models, islet cytoprotection by activated protein C (aPC) restores islet vascularization and protects graft function, suggesting that aPC triggers various lineages. aPC also prompts the release of endothelial MP that bear EPCR, its specific receptor. Microparticles (MP) are plasma membrane procoagulant vesicles, surrogate markers of stress and cellular effectors. We measured the cytoprotective effects of aPC on endothelial and insulin-secreting Rin-m5f ?-cells and its role in autocrine and paracrine MP-mediated cell crosstalk under conditions of oxidative stress. MP from aPC-treated primary endothelial (EC) or ?-cells were applied to H(2) O(2) -treated Rin-m5f. aPC activity was measured by enzymatic assay and ROS species by dihydroethidium. The capture of PKH26-stained MP and the expression of EPCR were probed by fluorescence microscopy and apoptosis by flow cytometry. aPC treatment enhanced both annexin A1 (ANXA1) and PAR-1 expression in EC and to a lesser extent in ?-cells. MP from aPC-treated EC (eM(aPC) ) exhibited high EPCR and annexin A1 content, protected ?-cells, restored insulin secretion and were captured by 80% of ? cells in a phosphatidylserine and ANXA1-dependent mechanism. eMP activated EPCR/PAR-1 and ANXA1/FPR2-dependent pathways and up-regulated the expression of EPCR, and of FPR2/ALX, the ANXA1 receptor. Cytoprotection was confirmed in H(2) O(2) -treated rat islets with increased viability (62% versus 48% H(2) O(2) ), reduced apoptosis and preserved insulin secretion in response to glucose elevation (16 versus 5 ng/ml insulin per 10 islets). MP may prove a promising therapeutic tool in the protection of transplanted islets. Twit Text FOAVip Endothelial microparticles released by activated protein C protect beta cells through EPCR/PAR1 and annexin A1/FPR2 pathways in islets ????J Cell Mol Med http://www.kidney.de/pget.php?&tw=1&pmid=28524456 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28524456 #FOAvip English Wikipedia <ref>{{Cite pmid|28524456 }}</ref> Endothelial microparticles released by activated protein C protect beta cells through EPCR/PAR1 and annexin A1/FPR2 pathways in islets #MMPMID28524456 Kreutter G ; Kassem M ; El Habhab A ; Baltzinger P ; Abbas M ; Boisrame-Helms J ; Amoura L ; Peluso J ; Yver B ; Fatiha Z ; Ubeaud-Sequier G ; Kessler L ; Toti F J Cell Mol Med 2017[Nov]; 21 (11 ): 2759-2772 PMID28524456 show ga Islet transplantation is associated with early ischaemia/reperfusion, localized coagulation and redox-sensitive endothelial dysfunction. In animal models, islet cytoprotection by activated protein C (aPC) restores islet vascularization and protects graft function, suggesting that aPC triggers various lineages. aPC also prompts the release of endothelial MP that bear EPCR, its specific receptor. Microparticles (MP) are plasma membrane procoagulant vesicles, surrogate markers of stress and cellular effectors. We measured the cytoprotective effects of aPC on endothelial and insulin-secreting Rin-m5f ?-cells and its role in autocrine and paracrine MP-mediated cell crosstalk under conditions of oxidative stress. MP from aPC-treated primary endothelial (EC) or ?-cells were applied to H(2) O(2) -treated Rin-m5f. aPC activity was measured by enzymatic assay and ROS species by dihydroethidium. The capture of PKH26-stained MP and the expression of EPCR were probed by fluorescence microscopy and apoptosis by flow cytometry. aPC treatment enhanced both annexin A1 (ANXA1) and PAR-1 expression in EC and to a lesser extent in ?-cells. MP from aPC-treated EC (eM(aPC) ) exhibited high EPCR and annexin A1 content, protected ?-cells, restored insulin secretion and were captured by 80% of ? cells in a phosphatidylserine and ANXA1-dependent mechanism. eMP activated EPCR/PAR-1 and ANXA1/FPR2-dependent pathways and up-regulated the expression of EPCR, and of FPR2/ALX, the ANXA1 receptor. Cytoprotection was confirmed in H(2) O(2) -treated rat islets with increased viability (62% versus 48% H(2) O(2) ), reduced apoptosis and preserved insulin secretion in response to glucose elevation (16 versus 5 ng/ml insulin per 10 islets). MP may prove a promising therapeutic tool in the protection of transplanted islets. |Animals [MESH] |Annexin A1/*genetics/metabolism [MESH] |Cell Line [MESH] |Cell-Derived Microparticles/*chemistry/metabolism [MESH] |Coronary Vessels/chemistry/cytology/metabolism [MESH] |Endothelial Cells/chemistry/cytology/metabolism [MESH] |Gene Expression Regulation [MESH] |Hydrogen Peroxide/antagonists & inhibitors/pharmacology [MESH] |Insulin-Secreting Cells/cytology/*drug effects/metabolism [MESH] |Islets of Langerhans/cytology/drug effects/metabolism [MESH] |Primary Cell Culture [MESH] |Protective Agents/pharmacology [MESH] |Protein C/*pharmacology [MESH] |Protein Serine-Threonine Kinases/*genetics/metabolism [MESH] |Rats [MESH] |Receptors, Endothelin/*genetics/metabolism [MESH] |Receptors, Lipoxin/*genetics/metabolism [MESH] |Signal Transduction [MESH] |Swine [MESH]Endothelial microparticles released by activated protein C protect bet(epmc).pdf DeepDyve Pubget Overpricing