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10.1111/jcmm.13204 http://scihub22266oqcxt.onion/10.1111/jcmm.13204 C5661257!5661257 !28597545     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\28597545 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       J+Cell+Mol+Med 2017 ; 21 (11 ): 2926-2936 Nephropedia Template TP {{tp|p=28597545 |t=2017. Cimifugin suppresses allergic inflammation by reducing epithelial derived initiative key factors via regulating tight junctions |pdf=|usr=}}{{28597545 }} gab.com Text Cimifugin suppresses allergic inflammation by reducing epithelial derived initiative key factors via regulating tight junctions JO: J Cell Mol Med http://www.kidney.de/pget.php?&tw=1&pmid=28597545 #FOAvip Cimifugin is a bioactive component of Saposhnikovia divaricata, a Chinese herb for treating allergy. Our previous studies demonstrated that cimifugin inhibited allergic inflammation efficiently. This study aims to determine the mechanism of cimifugin on epithelial cells in allergic inflammation. Mice were sensitized and challenged with FITC to establish type 2 atopic dermatitis (AD) model. The initial stage of AD model, in which mice were just sensitized with FITC, was established in vivo and immortalized human epidermal (HaCaT) cells were utilized in vitro. Initiative key cytokines, TSLP and IL-33, were measured by ELISA, the junctions in ECs were observed by electron microscopy and TJs (CLDN-1, occludin and CLDND1) were assessed by Western blot, immunohistochemistry and immunofluorescence. The results showed that TSLP and IL-33 were inhibited significantly by cimifugin in the initial stage of AD model. Simultaneously, cimifugin reduced the separated gap among the epithelial cells and increased the expression of TJs. Similar effects on TSLP/IL-33 and TJs were obtained in vitro. The effect of cimifugin on TSLP decreased significantly when expression of CLDN1 was interfered with siRNA and this implied cimifugin inhibits initiative cytokines through restoring TJs. Furthermore, cimifugin administered only in the initial stage obviously attenuated the ultimate allergic inflammation, which indicate that impacts of cimifugin in the initial stage on TSLP/IL-33 and TJs are sufficient for suppressing allergic inflammation. This study not only revealed the mechanisms of cimifugin, but also indicated the possibility of initiative key cytokines and TJs as therapeutic targets. Twit Text FOAVip Cimifugin suppresses allergic inflammation by reducing epithelial derived initiative key factors via regulating tight junctions ????J Cell Mol Med http://www.kidney.de/pget.php?&tw=1&pmid=28597545 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28597545 #FOAvip English Wikipedia <ref>{{Cite pmid|28597545 }}</ref> Cimifugin suppresses allergic inflammation by reducing epithelial derived initiative key factors via regulating tight junctions #MMPMID28597545 Wang X ; Jiang X ; Yu X ; Liu H ; Tao Y ; Jiang G ; Hong M J Cell Mol Med 2017[Nov]; 21 (11 ): 2926-2936 PMID28597545 show ga Cimifugin is a bioactive component of Saposhnikovia divaricata, a Chinese herb for treating allergy. Our previous studies demonstrated that cimifugin inhibited allergic inflammation efficiently. This study aims to determine the mechanism of cimifugin on epithelial cells in allergic inflammation. Mice were sensitized and challenged with FITC to establish type 2 atopic dermatitis (AD) model. The initial stage of AD model, in which mice were just sensitized with FITC, was established in vivo and immortalized human epidermal (HaCaT) cells were utilized in vitro. Initiative key cytokines, TSLP and IL-33, were measured by ELISA, the junctions in ECs were observed by electron microscopy and TJs (CLDN-1, occludin and CLDND1) were assessed by Western blot, immunohistochemistry and immunofluorescence. The results showed that TSLP and IL-33 were inhibited significantly by cimifugin in the initial stage of AD model. Simultaneously, cimifugin reduced the separated gap among the epithelial cells and increased the expression of TJs. Similar effects on TSLP/IL-33 and TJs were obtained in vitro. The effect of cimifugin on TSLP decreased significantly when expression of CLDN1 was interfered with siRNA and this implied cimifugin inhibits initiative cytokines through restoring TJs. Furthermore, cimifugin administered only in the initial stage obviously attenuated the ultimate allergic inflammation, which indicate that impacts of cimifugin in the initial stage on TSLP/IL-33 and TJs are sufficient for suppressing allergic inflammation. This study not only revealed the mechanisms of cimifugin, but also indicated the possibility of initiative key cytokines and TJs as therapeutic targets. |Animals [MESH] |Anti-Allergic Agents/isolation & purification/*pharmacology [MESH] |Apiaceae/chemistry [MESH] |Cell Line, Transformed [MESH] |Chromones/isolation & purification/*pharmacology [MESH] |Claudin-1/genetics/metabolism [MESH] |Claudins/genetics/metabolism [MESH] |Dermatitis, Atopic/chemically induced/*drug therapy/genetics/pathology [MESH] |Disease Models, Animal [MESH] |Fluorescein-5-isothiocyanate/administration & dosage [MESH] |Gene Expression Regulation [MESH] |Humans [MESH] |Immunoglobulins/*genetics/metabolism [MESH] |Inflammation [MESH] |Interleukin-33/*genetics/metabolism [MESH] |Keratinocytes/drug effects/metabolism/pathology [MESH] |Mice [MESH] |Mice, Inbred BALB C [MESH] |Occludin/genetics/metabolism [MESH] |Receptors, Cytokine/*genetics/metabolism [MESH] |Signal Transduction [MESH]Cimifugin suppresses allergic inflammation by reducing epithelial deri(epmc).pdf DeepDyve Pubget Overpricing