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10.1038/s41598-017-14230-1 http://scihub22266oqcxt.onion/10.1038/s41598-017-14230-1 C5660277!5660277 !29079724     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\29079724 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Sci+Rep 2017 ; 7 (1 ): 14199 Nephropedia Template TP {{tp|p=29079724 |t=2017. Ca(2+) protein alpha 1D of CaV1 3 regulates intracellular calcium concentration and migration of colon cancer cells through a non-canonical activity |pdf=|usr=}}{{29079724 }} gab.com Text Ca(2+) protein alpha 1D of CaV1 3 regulates intracellular calcium concentration and migration of colon cancer cells through a non-canonical activity JO: Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=29079724 #FOAvip It is generally accepted that voltage-gated Ca(2+) channels, CaV, regulate Ca(2+) homeostasis in excitable cells following plasma membrane depolarization. Here, we show that the Ca(2+) protein ?1D of CaV1.3 channel is overexpressed in colorectal cancer biopsies compared to normal tissues. Gene silencing experiments targeting ?1D reduced the migration and the basal cytosolic Ca(2+) concentration of HCT116 colon cancer cell line and modified the cytosolic Ca(2+) oscillations induced by the sodium/calcium exchanger NCX1/3 working in its reverse mode. Interestingly, NCX1/3 regulated membrane potential of HCT116 cells only when ?1D was silenced, and blocking NCX1/3 increased cytosolic Ca(2+) concentration and cell migration. However, membrane depolarization did not induce an increase in intracellular Ca(2+). Patch-clamp experiments clearly showed that the inward Ca(2+) current was absent. Finally, flow cytometry and immunofluorescence studies showed that ?1D protein was localized at the plasma membrane, in cytosol and cell nuclei. Altogether, we uncover a novel signaling pathway showing that ?1D is involved in the regulation of Ca(2+) homeostasis and cell migration by a mechanism independent of its plasma membrane canonical function but that involved plasma membrane Na(+)/Ca(2+) exchanger. Twit Text FOAVip Ca(2+) protein alpha 1D of CaV1 3 regulates intracellular calcium concentration and migration of colon cancer cells through a non-canonical activity ????Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=29079724 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=29079724 #FOAvip English Wikipedia <ref>{{Cite pmid|29079724 }}</ref> Ca(2+) protein alpha 1D of CaV1 3 regulates intracellular calcium concentration and migration of colon cancer cells through a non-canonical activity #MMPMID29079724 Fourbon Y ; Guéguinou M ; Félix R ; Constantin B ; Uguen A ; Fromont G ; Lajoie L ; Magaud C ; Lecomte T ; Chamorey E ; Chatelier A ; Mignen O ; Potier-Cartereau M ; Chantôme A ; Bois P ; Vandier C Sci Rep 2017[Oct]; 7 (1 ): 14199 PMID29079724 show ga It is generally accepted that voltage-gated Ca(2+) channels, CaV, regulate Ca(2+) homeostasis in excitable cells following plasma membrane depolarization. Here, we show that the Ca(2+) protein ?1D of CaV1.3 channel is overexpressed in colorectal cancer biopsies compared to normal tissues. Gene silencing experiments targeting ?1D reduced the migration and the basal cytosolic Ca(2+) concentration of HCT116 colon cancer cell line and modified the cytosolic Ca(2+) oscillations induced by the sodium/calcium exchanger NCX1/3 working in its reverse mode. Interestingly, NCX1/3 regulated membrane potential of HCT116 cells only when ?1D was silenced, and blocking NCX1/3 increased cytosolic Ca(2+) concentration and cell migration. However, membrane depolarization did not induce an increase in intracellular Ca(2+). Patch-clamp experiments clearly showed that the inward Ca(2+) current was absent. Finally, flow cytometry and immunofluorescence studies showed that ?1D protein was localized at the plasma membrane, in cytosol and cell nuclei. Altogether, we uncover a novel signaling pathway showing that ?1D is involved in the regulation of Ca(2+) homeostasis and cell migration by a mechanism independent of its plasma membrane canonical function but that involved plasma membrane Na(+)/Ca(2+) exchanger. |*Cell Movement [MESH] |Active Transport, Cell Nucleus [MESH] |Calcium Channels, L-Type/*chemistry/*metabolism [MESH] |Calcium/*metabolism [MESH] |Cell Membrane/metabolism [MESH] |Cell Nucleus/metabolism [MESH] |Colonic Neoplasms/*metabolism/*pathology/physiopathology [MESH] |Cytosol/metabolism [MESH] |Electrophysiological Phenomena [MESH] |Endoplasmic Reticulum/metabolism [MESH] |Gene Expression Regulation, Neoplastic [MESH] |HCT116 Cells [MESH] |Humans [MESH] |Intracellular Space/*metabolism [MESH]Ca(2+) protein alpha 1D of CaV1 3 regulates intracellular calcium conc(epmc).pdf DeepDyve Pubget Overpricing