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Propofol inhibits the release of interleukin-6, 8 and tumor necrosis factor-?
correlating with high-mobility group box 1 expression in
lipopolysaccharides-stimulated RAW 264 7 cells
#MMPMID29073894
Jia J
; Sun Y
; Hu Z
; Li Y
; Ruan X
BMC Anesthesiol
2017[Oct]; 17
(1
): 148
PMID29073894
show ga
BACKGROUND: Studies have found that propofol can inhibit endotoxin-induced
monocyte-macrophages to produce various inflammatory factors. This study is to
disclose whether the propofol affects the expression of high-mobility group box 1
(HMGB1) in lipopolysaccharides (LPS)-stimulated RAW 264.7 cells and the release
of interleukin-6 (IL-6), 8 (IL-8) and tumor necrosis factor-? (TNF-?). METHODS:
RAW 264.7 cells were divided into four groups for intervention. After culturing
for 16 h, the cells and culture supernatants were collected. The expression of
HMGB1 in RAW 264.7 cells was detected by Western blot. The levels of IL-6, IL-8
and TNF-? in supernatants of cells were determined by enzyme-linked immunosorbent
assay (ELISA). RESULTS: Stimulation of LPS increased the expression of HMGB1 and
promoted the release of IL-6, IL-8 and TNF-? in supernatants of RAW 264.7 cells
(p < 0.05); however, propofol down-regulated the expression of LPS-stimulated
HMGB1 and reduced the LPS-stimulated releases of IL-6, IL-8 and TNF-? in
supernatants of RAW 264.7 cells (p < 0.05). Moreover, the releases of IL-6, IL-8
and TNF-? intimately correlated with the expression of HMGB1 in this process
(p < 0.05). CONCLUSION: Propofol inhibited the releases of IL-6, IL-8 and TNF-?
in LPS-stimulated RAW 264.7 cells, and the levels of IL-6, IL-8 and TNF-?
intimately correlated with the expression of HMGB1, which indicating that
propofol may prevent inflammatory responses through reducing the releases of
these cytokines and inflammatory mediators.
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