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2017 ; 14
(5
): 4201-4207
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Mechanism of myocardial ischemia/reperfusion-induced acute kidney injury through
DJ-1/Nrf2 pathway in diabetic rats
#MMPMID29104636
Sun Q
; Shen ZY
; Duan WN
; Meng QT
; Xia ZY
Exp Ther Med
2017[Nov]; 14
(5
): 4201-4207
PMID29104636
show ga
The objective of the present study was to investigate acute kidney injury (AKI)
induced by myocardial ischemia/reperfusion (MIR) in diabetic rats and elucidate
its underlying mechanism. A rat model of MIR was established by left anterior
descending coronary artery occlusion for 30 min, followed by reperfusion for 2 h.
Rats were randomly divided into four groups: i) Sham group, ii) sham + MIR group,
iii) diabetic group and iv) diabetes + MIR group. Myocardial injury was detected
by plasma creatine kinase isoenzyme MB and lactate dehydrogenase assays. AKI
induced by MIR in diabetic rats was characterized by increases in cystatin C and
?2-microglobulin levels. Oxidative stress injury was accompanied by an increase
of malondialdehyde levels and a decrease of total antioxidative capacity in the
renal tissues. Immunohistochemistry and western blot analysis demonstrated that
the expression of DJ-1 and nuclear factor erythroid 2-related factor 2 (Nrf2)
were significantly increased in the diabetes + MIR group compared with that in
the sham + MIR and diabetic groups. Taken together, these results suggested that
AKI induced by MIR in diabetic rats may be associated with activation of the
DJ-1/Nrf2 pathway.