Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=29100368
&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests
in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215
Glis family proteins are differentially implicated in the cellular reprogramming
of human somatic cells
#MMPMID29100368
Lee SY
; Noh HB
; Kim HT
; Lee KI
; Hwang DY
Oncotarget
2017[Sep]; 8
(44
): 77041-77049
PMID29100368
show ga
The ground-breaking discovery of the reprogramming of somatic cells into
pluripotent cells, termed induced pluripotent stem cells (iPSCs), was
accomplished by delivering 4 transcription factors, Oct4, Sox2, Klf4, and c-Myc,
into fibroblasts. Since then, several efforts have attempted to unveil other
factors that are directly implicated in or might enhance reprogramming.
Importantly, a number of transcription factors are reported to retain
reprogramming activity. A previous study suggested Gli-similar 1 (Glis1) as a
factor that enhances the reprogramming of fibroblasts during iPSC generation.
However, the implication of other Glis members, including Glis2 and Glis3
(variants 1 and 2), in cellular reprogramming remains unknown. In this study, we
investigated the potential involvement of human Glis family proteins, including
hGlis1-3, in cellular reprogramming. Our results demonstrate that hGlis1, which
is reported to reprogram human fibroblasts, promotes the reprogramming of human
adipose-derived stromal cells (hADSCs), indicating that the reprogramming
activity of Glis1 is not cell type-specific. Strikingly, hGlis3 promoted the
reprogramming of hADSCs as efficiently as hGlis1. On the contrary, hGlis2 showed
a strong negative effect on reprogramming. Together, our results reveal clear
differences in the cellular reprogramming activity among Glis family members and
provide valuable insight into the development of a new reprogramming strategy
using Glis family proteins.
free
free
free
