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10.1038/pr.2017.157 http://scihub22266oqcxt.onion/10.1038/pr.2017.157 C5645264!5645264 !28665931     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\28665931 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Pediatr+Res 2017 ; 82 (5 ): 855-862 Nephropedia Template TP {{tp|p=28665931 |t=2017. Foxd1 is an upstream regulator of the renin-angiotensin system during metanephric kidney development |pdf=|usr=}}{{28665931 }} gab.com Text Foxd1 is an upstream regulator of the renin-angiotensin system during metanephric kidney development JO: Pediatr Res http://www.kidney.de/pget.php?&tw=1&pmid=28665931 #FOAvip BackgroundWe tested the hypothesis that Foxd1, a transcription factor essential for normal kidney development, is an upstream regulator of the renin-angiotensin system (RAS) during ureteric bud (UB)-branching morphogenesis.MethodsUB branching, RAS gene, and protein expression were studied in embryonic mouse kidneys. RAS mRNA expression was studied in mesenchymal MK4 cells.ResultsThe number of UB tips was reduced in Foxd1(-/-) compared with that in Foxd1(+/+) metanephroi on embryonic day E12.5 (14±2.1 vs. 28±1.3, P<0.05). Quantitative real-time reverse-transcription polymerase chain reaction (qRT-PCR) demonstrated that renin, angiotensin I-converting enzyme (ACE), and angiotensin (Ang) II receptor type 1 (AT(1)R) mRNA levels were decreased in Foxd1(-/-) compared with those in Foxd1(+/+) E14.5 metanephroi. Western blot analysis and immunohistochemistry showed decreased expression of AGT and renin proteins in Foxd1(-/-) metanephroi compared with that in Foxd1(+/+) metanephroi. Foxd1 overexpression in mesenchymal MK4 cells in vitro increased renin, AGT, ACE, and AT(1)R mRNA levels. Exogenous Ang II stimulated UB branching equally in whole intact E12.5 Foxd1(-/-) and Foxd1(+/+) metanephroi grown ex vivo (+364±21% vs. +336±18%, P=0.42).ConclusionWe conclude that Foxd1 is an upstream positive regulator of RAS during early metanephric development and propose that the cross-talk between Foxd1 and RAS is essential in UB-branching morphogenesis. Twit Text FOAVip Foxd1 is an upstream regulator of the renin-angiotensin system during metanephric kidney development ????Pediatr Res http://www.kidney.de/pget.php?&tw=1&pmid=28665931 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28665931 #FOAvip English Wikipedia <ref>{{Cite pmid|28665931 }}</ref> Foxd1 is an upstream regulator of the renin-angiotensin system during metanephric kidney development #MMPMID28665931 Song R ; Lopez MLSS ; Yosypiv IV Pediatr Res 2017[Nov]; 82 (5 ): 855-862 PMID28665931 show ga BackgroundWe tested the hypothesis that Foxd1, a transcription factor essential for normal kidney development, is an upstream regulator of the renin-angiotensin system (RAS) during ureteric bud (UB)-branching morphogenesis.MethodsUB branching, RAS gene, and protein expression were studied in embryonic mouse kidneys. RAS mRNA expression was studied in mesenchymal MK4 cells.ResultsThe number of UB tips was reduced in Foxd1(-/-) compared with that in Foxd1(+/+) metanephroi on embryonic day E12.5 (14±2.1 vs. 28±1.3, P<0.05). Quantitative real-time reverse-transcription polymerase chain reaction (qRT-PCR) demonstrated that renin, angiotensin I-converting enzyme (ACE), and angiotensin (Ang) II receptor type 1 (AT(1)R) mRNA levels were decreased in Foxd1(-/-) compared with those in Foxd1(+/+) E14.5 metanephroi. Western blot analysis and immunohistochemistry showed decreased expression of AGT and renin proteins in Foxd1(-/-) metanephroi compared with that in Foxd1(+/+) metanephroi. Foxd1 overexpression in mesenchymal MK4 cells in vitro increased renin, AGT, ACE, and AT(1)R mRNA levels. Exogenous Ang II stimulated UB branching equally in whole intact E12.5 Foxd1(-/-) and Foxd1(+/+) metanephroi grown ex vivo (+364±21% vs. +336±18%, P=0.42).ConclusionWe conclude that Foxd1 is an upstream positive regulator of RAS during early metanephric development and propose that the cross-talk between Foxd1 and RAS is essential in UB-branching morphogenesis. |*Renin-Angiotensin System/genetics [MESH] |Angiotensinogen/genetics/metabolism [MESH] |Animals [MESH] |Cell Line [MESH] |Forkhead Transcription Factors/deficiency/genetics/*metabolism [MESH] |Gene Expression Regulation, Developmental [MESH] |Genotype [MESH] |Kidney/embryology/*metabolism [MESH] |Mice, Knockout [MESH] |Morphogenesis [MESH] |Peptidyl-Dipeptidase A/genetics/metabolism [MESH] |Phenotype [MESH] |Receptor, Angiotensin, Type 1/genetics/metabolism [MESH] |Renin/metabolism [MESH] |Signal Transduction [MESH] |Time Factors [MESH]Foxd1 is an upstream regulator of the renin-angiotensin system during (epmc).pdf DeepDyve Pubget Overpricing