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10.1093/jisesa/ieu094

http://scihub22266oqcxt.onion/10.1093/jisesa/ieu094
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C5634058!5634058!25525100
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suck abstract from ncbi


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pmid25525100      J+Insect+Sci 2014 ; 14 (ä): ä
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  • Mechanism of Entomotoxicity of the Concanavalin A in Rhopalosiphum padi (Hemiptera: Aphididae) #MMPMID25525100
  • Sprawka I; Go?awska S; Parzych T; Go?awski A; Czerniewicz P; Sytykiewicz H
  • J Insect Sci 2014[]; 14 (ä): ä PMID25525100show ga
  • The toxicity effect of Concanavalin A ( Canavalia ensiformis lectin, ConA) to bird cherry-oat aphid, Rhopalosiphum padi L. (Hemiptera: Aphididae), was investigated in the laboratory by using artificial diets containing ConA concentrations. Bird cherry-oat aphid performance was affected by the presence of Con A in artificial diets. The lectin added into the liquid diet increased the prereproductive period, mortality, and the average time of generation development ( T ) and decreased fecundity and the intrinsic rate of natural increase ( rm ). In attempt to unravel the mode of action of ConA, the interaction of the lectin with insect gut and the effect of ConA on feeding behavior were investigated. Extract of gut of treated grain aphid demonstrated DNA fragmentation, and this was accompanied with an increase in caspase 3 activity. Moreover, addition of ConA to the sucrose?agarose gels reduced salivation and passive ingestion of fluids from the gel. The results indicate that the insecticidal activity of ConA on R. padi may involve effects on death of the gut epithelial cells and effects on feeding behavior. This can be employed to create plants that are resistant to aphids.
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