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10.1038/s41566-017-0001-7

http://scihub22266oqcxt.onion/10.1038/s41566-017-0001-7
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C5624503!5624503!28983324
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suck abstract from ncbi


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pmid28983324      Nat+Photonics 2017 ; 11 (10): 657-63
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  • Giant light-harvesting nanoantenna for single-molecule detection in ambient light #MMPMID28983324
  • Trofymchuk K; Reisch A; Didier P; Fras F; Gilliot P; Mely Y; Klymchenko AS
  • Nat Photonics 2017[Oct]; 11 (10): 657-63 PMID28983324show ga
  • Here, we explore the enhancement of single molecule emission by polymeric nano-antenna that can harvest energy from thousands of donor dyes to a single acceptor. In this nano-antenna, the cationic dyes are brought together in very close proximity using bulky counterions, thus enabling ultrafast diffusion of excitation energy (?30 fs) with minimal losses. Our 60-nm nanoparticles containing >10,000 rhodamine-based donor dyes can efficiently transfer energy to 1-2 acceptors resulting in an antenna effect of ~1,000. Therefore, single Cy5-based acceptors become 25-fold brighter than quantum dots QD655. This unprecedented amplification of the acceptor dye emission enables observation of single molecules at illumination powers (1-10 mW cm-2) that are >10,000-fold lower than typically required in single-molecule measurements. Finally, using a basic setup, which includes a 20X air objective and a sCMOS camera, we could detect single Cy5 molecules by simply shining divergent light on the sample at powers equivalent to sunlight.
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