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10.3390/cells6030017 http://scihub22266oqcxt.onion/10.3390/cells6030017 C5617963!5617963!28665306     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Cells 2017 ; 6 (3): ä Nephropedia Template TP {{tp|p=28665306|t=2017. Standard Immunohistochemical Assays to Assess Autophagy in Mammalian Tissue |pdf=|usr=}}{{28665306}} gab.com Text Standard Immunohistochemical Assays to Assess Autophagy in Mammalian Tissue JO: Cells http://www.kidney.de/pget.php?&tw=1&pmid=28665306 #FOAvip Autophagy is a highly conserved lysosomal degradation pathway with major impact on diverse human pathologies. Despite the development of different methodologies to detect autophagy both in vitro and in vivo, monitoring autophagy in tissue via immunohistochemical techniques is hampered due to the lack of biomarkers. Immunohistochemical detection of a punctate pattern of ATG8/MAP1LC3 proteins is currently the most frequently used approach to detect autophagy in situ, but it depends on a highly sensitive detection method and is prone to misinterpretation. Moreover, reliable MAP1LC3 immunohistochemical staining requires correct tissue processing and high-quality, isoform-specific antibodies. Immunohistochemical analysis of other autophagy-related protein targets such as SQSTM1, ubiquitin, ATG5 or lysosomal proteins is not recommended as marker for autophagic activity in tissue for multiple reasons including aspecific labeling of cellular structures and a lack of differential protein expression during autophagy initiation. To better understand the role of autophagy in human disease, novel biomarkers for visualization of the autophagic process with standard histology techniques are urgently needed. Twit Text FOAVip Standard Immunohistochemical Assays to Assess Autophagy in Mammalian Tissue ????Cells http://www.kidney.de/pget.php?&tw=1&pmid=28665306 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28665306 #FOAvip English Wikipedia <ref>{{Cite pmid|28665306}}</ref> Standard Immunohistochemical Assays to Assess Autophagy in Mammalian Tissue #MMPMID28665306 Martinet W; Roth L; De Meyer GRY Cells 2017[Sep]; 6 (3): ä PMID28665306show ga Autophagy is a highly conserved lysosomal degradation pathway with major impact on diverse human pathologies. Despite the development of different methodologies to detect autophagy both in vitro and in vivo, monitoring autophagy in tissue via immunohistochemical techniques is hampered due to the lack of biomarkers. Immunohistochemical detection of a punctate pattern of ATG8/MAP1LC3 proteins is currently the most frequently used approach to detect autophagy in situ, but it depends on a highly sensitive detection method and is prone to misinterpretation. Moreover, reliable MAP1LC3 immunohistochemical staining requires correct tissue processing and high-quality, isoform-specific antibodies. Immunohistochemical analysis of other autophagy-related protein targets such as SQSTM1, ubiquitin, ATG5 or lysosomal proteins is not recommended as marker for autophagic activity in tissue for multiple reasons including aspecific labeling of cellular structures and a lack of differential protein expression during autophagy initiation. To better understand the role of autophagy in human disease, novel biomarkers for visualization of the autophagic process with standard histology techniques are urgently needed. äStandard Immunohistochemical Assays to Assess Autophagy in Mammalian T(epmc).pdf DeepDyve Pubget Overpricing