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10.1038/nbt.3770 http://scihub22266oqcxt.onion/10.1038/nbt.3770 C5617104!5617104!28092656     free     free     free Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nat+Biotechnol 2017 ; 35 (2): 164-72 Nephropedia Template TP {{tp|p=28092656|t=2017. Influence of node abundance on signaling network state and dynamics analyzed by mass cytometry |pdf=|usr=}}{{28092656}} gab.com Text Influence of node abundance on signaling network state and dynamics analyzed by mass cytometry JO: Nat Biotechnol http://www.kidney.de/pget.php?&tw=1&pmid=28092656 #FOAvip Signaling networks are key regulators of cellular function. Although the concentrations of signaling proteins are perturbed in disease states, such as cancer, and are modulated by drug therapies, our understanding of how such changes shape the properties of signaling networks is limited. Here we couple mass cytometry-based single-cell analysis with overexpression of tagged signaling proteins to study the dependence of signaling relationships and dynamics on protein node abundance. Focusing on the epidermal growth factor receptor (EGFR) signaling network in HEK293T cells, we analyze 20 signaling proteins during a one hour EGF stimulation time course using a panel of 35 antibodies. Data analysis with BP-R2, a measure that quantifies complex signaling relationships, reveals abundance-dependent network states and identifies novel signaling relationships. Further, we show that upstream signaling proteins have abundance-dependent effects on downstream signaling dynamics. Our approach elucidates the influence of node abundance on signal transduction networks and will further our understanding of signaling in health and disease. Twit Text FOAVip Influence of node abundance on signaling network state and dynamics analyzed by mass cytometry ????Nat Biotechnol http://www.kidney.de/pget.php?&tw=1&pmid=28092656 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28092656 #FOAvip English Wikipedia <ref>{{Cite pmid|28092656}}</ref> Influence of node abundance on signaling network state and dynamics analyzed by mass cytometry #MMPMID28092656 Lun XK; Zanotelli VR; Wade JD; Schapiro D; Tognetti M; Dobberstein N; Bodenmiller B Nat Biotechnol 2017[Feb]; 35 (2): 164-72 PMID28092656show ga Signaling networks are key regulators of cellular function. Although the concentrations of signaling proteins are perturbed in disease states, such as cancer, and are modulated by drug therapies, our understanding of how such changes shape the properties of signaling networks is limited. Here we couple mass cytometry-based single-cell analysis with overexpression of tagged signaling proteins to study the dependence of signaling relationships and dynamics on protein node abundance. Focusing on the epidermal growth factor receptor (EGFR) signaling network in HEK293T cells, we analyze 20 signaling proteins during a one hour EGF stimulation time course using a panel of 35 antibodies. Data analysis with BP-R2, a measure that quantifies complex signaling relationships, reveals abundance-dependent network states and identifies novel signaling relationships. Further, we show that upstream signaling proteins have abundance-dependent effects on downstream signaling dynamics. Our approach elucidates the influence of node abundance on signal transduction networks and will further our understanding of signaling in health and disease. äInfluence of node abundance on signaling network state and dynamics an(epmc).pdf DeepDyve Pubget Overpricing