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2016 ; 3
(ä): 733-739
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In vitro mutagenicity assay (Ames test) and phytochemical characterization of
seeds oil of Helianthus annuus Linné (sunflower)
#MMPMID28959599
de Mello Silva Oliveira N
; Reis Resende M
; Alexandre Morales D
; de Ragăo Umbuzeiro G
; Boriollo MFG
Toxicol Rep
2016[]; 3
(ä): 733-739
PMID28959599
show ga
The objective of this research was to investigate the genotoxic potential of the
oil of H. annuus L. (sunflower) seeds via the Ames test as well as its oxidative
properties and lipid composition. The pre-incubation method, system metabolic
activation (S9 fraction) and five S. typhimurium strains (TA97, TA98, TA100,
TA1535 and TA102) were employed for the Ames test. The oxidative stability and
fatty acid composition were analyzed by standard methods and gas chromatography.
A revertant analysis showed no significant differences between the treatment
doses (10-200 ?l/plate) and the negative controls, regardless of S9(+) and S9(-),
and included all of the S. typhimurium strains. Chromatographic analysis showed
high levels of polyunsaturated fatty acids, followed by monounsaturated,
saturated and total trans-isomers. Among the polyunsaturated, monounsaturated and
saturated fatty acids, linoleic, oleic and palmitic acids predominated. The
results suggest that the sunflower oil is not genotoxic as indicated by
frameshift mutations and base pair substitutions regardless of the treatment
dose, but shows dose-dependent toxicity. The oxidative properties of the
sunflower oil were consistent with the requirements of national and international
standards. However, its composition could also indicate phytotherapeutic
properties.