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2017 ; 6
(Spec Iss 2
): S0072
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Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry: Mechanistic
Studies and Methods for Improving the Structural Identification of Carbohydrates
#MMPMID28959517
Lai YH
; Wang YS
Mass Spectrom (Tokyo)
2017[]; 6
(Spec Iss 2
): S0072
PMID28959517
show ga
Although matrix-assisted laser desorption/ionization (MALDI) mass spectrometry is
one of the most widely used soft ionization methods for biomolecules, the lack of
detailed understanding of ionization mechanisms restricts its application in the
analysis of carbohydrates. Structural identification of carbohydrates achieved by
MALDI mass spectrometry helps us to gain insights into biological functions and
pathogenesis of disease. In this review, we highlight mechanistic details of
MALDI, including both ionization and desorption. Strategies to improve the ion
yield of carbohydrates are also reviewed. Furthermore, commonly used
fragmentation methods to identify the structure are discussed.